Bibliographic Details
| Title: |
KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation. |
| Authors: |
Campbell, Mel, Tadashi Watanabe, Kazushi Nakano, Davis, Ryan R., Yuanzhi Lyu, Tepper, Clifford G., Durbin-Johnson, Blythe, Masahiro Fujimuro, Yoshihiro Izumiya |
| Source: |
Nature Communications; 1/4/2018, Vol. 9 Issue 1, p1-14, 14p |
| Subject Terms: |
GENETIC regulation, KAPOSI'S sarcoma-associated herpesvirus, EPISOMES, RNA polymerase II, GENE regulatory networks |
| Abstract: |
The three-dimensional structure of chromatin organized by genomic loops facilitates RNA polymerase II access to distal promoters. The Kaposi’s sarcoma-associated herpesvirus (KSHV) lytic transcriptional program is initiated by a single viral transactivator, K-Rta. Here we report the KSHV genomic structure and its relationship with K-Rta recruitment sites using Capture Hi–C analyses. High-resolution 3D viral genomic maps identify a number of direct physical, long-range, and dynamic genomic interactions. Mutant KSHV chromosomes harboring point mutations in the K-Rta responsive elements (RE) significantly attenuate not only the directly proximate downstream gene, but also distal gene expression in a domain-specific manner. Genomic loops increase in the presence of K-Rta, while abrogation of K-Rta binding impairs the formation of inducible genomic loops, decreases the expression of genes networked through the looping, and diminishes KSHV replication. Our study demonstrates that genomic architectural dynamics plays an essential role in herpesvirus gene expression. [ABSTRACT FROM AUTHOR] |
|
Copyright of Nature Communications is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
