Bibliographic Details
| Title: |
Technical Feasibility of a Murine Model of Sleeve Gastrectomy with Ileal Transposition. |
| Authors: |
Ying, Lee D., Breuer, Gregory A., Hubbard, Matthew O., Nadzam, Geoffrey S., Hwa, John, Martin, Kathleen A. |
| Source: |
Obesity Surgery; Feb2019, Vol. 29 Issue 2, p593-600, 8p |
| Subject Terms: |
WEIGHT loss, RATS |
| Abstract: |
Background: Sleeve gastrectomy with ileal transposition has been shown to be superior to sleeve gastrectomy alone for promoting weight loss in rat and porcine models. The absence of a mouse model for this procedure has impeded efforts to understand the molecular physiology underlying its efficacy. This study demonstrates the long-term survivability of sleeve gastrectomy with ileal transposition in mice.Materials and Methods: In this study of technical feasibility, a sleeve gastrectomy with ileal transposition (SGIT), sleeve gastrectomy (SG), or sham surgery (SH) was performed on 7- to 8-week-old C57Bl/6J mice (n = 8 for each). To evaluate long-term survivability, mice were placed on an obesogenic diet and weighed weekly for 10 weeks. The intestinal identity of the transposed segment was assessed with gene expression analysis of duodenal-, jejunal-, and ileal-specific hormones using quantitative polymerase chain reaction.Results: Overall, SGIT better prevented weight gain than the SG or sham procedures (10-week post-operative weight: SH 45.3 ± 1.0 g, SG 41.25 ± 1.6 g, SGIT 35.4 ± 0.8 g). Gene expression pattern analysis of three markers of intestinal identity (gastrin, cholecystokinin, and peptide YY) suggests that the ileal identity of the transposed segment is maintained 10 weeks after transposition.Conclusions: We demonstrate for the first time a reproducible mouse model of sleeve gastrectomy with ileal transposition. Future studies utilizing this model will expand our understanding of the molecular pathways through which the hindgut regulates satiety. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
