| Title: |
The Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination. |
| Authors: |
Heitzeberg, Fabian, Chen, I-peng, Hartung, Frank, Orel, Nadiya, Angelis, Karel J., Puchta, Holger |
| Source: |
Plant Journal; Jun2004, Vol. 38 Issue 6, p954-968, 15p |
| Subject Terms: |
ARABIDOPSIS, MUTAGENESIS, DNA damage, BLEOMYCIN, MITOMYCIN C, PLANT cells & tissues |
| Abstract: |
Rad17 is involved in DNA checkpoint control in yeast and human cells. A homologue of this gene as well as other genes of the pathway (the 9-1-1 complex) are present in Arabidopsis and share conserved sequence domains with their yeast and human counterparts. DNA-damaging agents induce At RAD17 transcriptionally. At RAD17 mutants show increased sensitivity to the DNA-damaging chemicals bleomycin and mitomycin C (MMC), which can be reversed by complementation, suggesting that the loss of function of Rad17 disturbs DNA repair in plant cells. Our results are further confirmed by the phenotype of a mutant of the 9-1-1 complex (Rad9), which is also sensitive to the same chemicals. At RAD9 and At RAD17 seem to be epistatic as the double mutant is not more sensitive to the chemicals than the single mutants. The mutants show a delay in the general repair of double-strand breaks (DSBs). However, frequencies of intrachromosomal homologous recombination (HR) are enhanced. Nevertheless, the mutants are proficient for a further induction of HR by genotoxic stresses. Our results indicate that a mutant Rad17 pathway is associated with a general deregulation of DNA repair, which seems to be correlated with a deficiency in non-homologous DSB repair. [ABSTRACT FROM AUTHOR] |
|
Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
