| Title: |
Whole-exome sequencing identifies a potential TTN mutation in a multiplex family with inguinal hernia. |
| Authors: |
Metspalu, A., Mihailov, E., Nikopensius, T., Reigo, A., Kals, M., Milani, L., Nikkolo, C., Seepter, H., Aruaas, K. |
| Source: |
Hernia; Feb2017, Vol. 21 Issue 1, p95-100, 6p |
| Subject Terms: |
HERNIA, INGUINAL hernia, GENETICS, EXOMES, HERNIA treatment, GENETIC mutation, GENEALOGY, GENETIC techniques, GENOMES, SEQUENCE analysis |
| Abstract: |
Purpose: Inguinal hernia repair is one of the most common procedures in general surgery. Males are seven times more likely than females to develop a hernia and have a 27 % lifetime 'risk' of inguinal hernia repair. Several studies have demonstrated that a positive family history is an important risk factor for the development of primary inguinal hernia, which indicates that genetic factors may play important roles in the etiology of the disease. So far, the contribution of genetic factors and underlying mechanisms for inguinal hernia remain largely unknown. The aim of this study was to investigate a multiplex Estonian family with inguinal hernia across four generations.Methods: The whole-exome sequencing was carried out in three affected family members and subsequent mutation screening using Sanger sequencing was performed in ten family members (six affected and four unaffected).Results: Whole-exome sequencing in three affected family members revealed a heterozygous missense mutation c.88880A>C (p.Lys29627Thr; RefSeq NM_001256850.1) in the highly conserved myosin-binding A-band of the TTN gene. Sanger sequencing demonstrated that this mutation cosegregated with the disease in this family and was not present in ethnically matched control subjects.Conclusion: We report that missense variant in the A-band of TTN is the strongest candidate mutation for autosomal-dominant inguinal hernia with incomplete penetrance. [ABSTRACT FROM AUTHOR] |
|
Copyright of Hernia is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
