Bibliographic Details
| Title: |
First look at RNA in L-configuration. |
| Authors: |
Vallazza, M., Perbandt, M., Klussmann, S., Rypniewski, W., Einspahr, H. M., Erdmann, V. A., Betzel, Ch. |
| Source: |
Acta Crystallographica: Section D (Wiley-Blackwell); Jan2004, Vol. 60 Issue 1, p1-7, 7p |
| Subject Terms: |
NUCLEOTIDE sequence, OLIGONUCLEOTIDES, BIODEGRADATION, DRUG development, CRYSTALLIZATION, NUCLEIC acids |
| Abstract: |
Nucleic acid molecules in the mirror image or L-configuration are unknown in nature and are extraordinarily resistant to biological degradation. The identification of functional L-oligonucleotides called Spiegelmers offers a novel approach for drug discovery based on RNA. The sequence r(CUGGGCGG).r(CCGCCUGG) was chosen as a model system for structural analysis of helices in the L-configuration as the structure of the D-form of this sequence has previously been determined in structural studies of 5S RNA domains, in particular domain E of the Thermus flavus 5S rRNA [Perbandt et al. (2001), Acta Cryst. D57, 219-224]. Unexpectedly, the results of crystallization trials showed little similarity between the D- and the L-forms of the duplex in either the crystallization hits or the diffraction performance. The crystal structure of this L-RNA duplex has been determined at 1.9 A resolution with R[subwork] and R[subfree] of 23.8 and 28.6%, respectively. The crystals belong to space group R32, with unit-cell parameters a = 45.7, c = 264.6 A. Although there are two molecules in the asymmetric unit rather than one, the structure of the L-form arranges helical pairs in a head-to-tail fashion to form pseudo-continuous infinite helices in the crystal as in the D-form. On the other hand, the wobble-like G.C(+) base pair seen in the D-RNA analogue does not appear in the L-RNA duplex, which forms a regular double-helical structure with typical Watson-Crick base pairing. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
