Bibliographic Details
| Title: |
Microsatellite Marker Development by Multiplex Ion Torrent PGM Sequencing: a Case Study of the Endangered Odorrana narina Complex of Frogs. |
| Authors: |
TAKESHI IGAWA, MASAFUMI NOZAWA, MAI NAGAOKA, SHOHEI KOMAKI, SHOHEI OUMI, TAMOTSU FUJII, MASAYUKI SUMIDA |
| Source: |
Journal of Heredity; Jan/Feb2015, Vol. 106 Issue 1, p131-137, 7p |
| Subject Terms: |
MICROSATELLITE repeats, AMPHIBIAN conservation, ENDANGERED species, GENOTYPES, NUCLEIC acid isolation methods |
| Abstract: |
The endangered Ryukyu tip-nosed frog Odorrana nanna and its related species, Odorrana amamiensis, Odorrana suprananna, and Odorrana utsunomiyaorum, belong to the family Ramdae and are endemically distributed in Okinawa (O. nanna), Amami and Tokunoshima (O. amamiensis), and Ishigaki and Inomote (O. supranarina and O. utsunomiyaorum) Islands. Because of varying distribution patterns, this species complex is an intrinsic model for speciation and adaptation. For effective conservation and molecular ecological studies, further genetic information is needed. For rapid, cost-effective development of several micro-satellite markers for these and 2 other species, we used next-generation sequencing technology of Ion Torrent PGMTM Distribution patterns of repeat motifs of microsatellite loci in these modern frog species (Neobatrachia) were similarly skewed. We isolated and characterized 20 new microsatellite loci of O. nanna and validated cross-amplification in the three-related species. Seventeen, 16, and 13 loci were cross-amplified in O. mammas, Q supranarina, and O. utsunomiyaorum, respectively, reflecting close genetic relationships between them. Mean number of alleles and expected heterozygosity of newly isolated loci varied depending on the size of each inhabited island. Our findings suggested the suitability of Ion Torrent PGMTM for microsatellite marker development. The new markers developed for the O. narina complex will be applicable in conservation genetics and molecular ecological studies. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
