Bibliographic Details
| Title: |
Polydopamine-Functionalization of Graphene Oxide to Enable Dual Signal Amplification for Sensitive Surface Plasmon Resonance Imaging Detection of Biomarker. |
| Authors: |
Weihua Hu1,2,3,4 whhu@swu.edu.cn, Guangli He1,2,3, Huanhuan Zhang1,2,3, Xiaoshuai Wu1,2,3, Jialin Li1,2,3, Zhiliang Zhao1,2,3, Yan Qiao1,2,3, Zhisong Lu1,2,3, Yang Liu5, Chang Ming Li1,2,3 |
| Source: |
Analytical Chemistry. 5/6/2014, Vol. 86 Issue 9, p4488-4493. 6p. |
| Subject Terms: |
*GRAPHENE oxide, *BLOOD plasma, *CHEMICAL reactions, *SERUM, *RESONANCE |
| Abstract: |
Surface plasmon resonance imaging (SPRi) is one of the powerful tools for immunoassays with advantages of label-free, real-time, and high-throughput; however, it often suffers from limited sensitivity. Herein we report a dual signal amplification strategy utilizing polydopamine (PDA) functionalization of reduced graphene oxide (PDA-rGO) nanosheets for sensitive SPRi immunoassay in serum. The PDA-rGO nanosheet is synthesized by oxidative polymerization of dopamine in a gentle alkaline solution in the presence of graphene oxide (GO) sheets and then is antibody-conjugated via a spontaneous reaction between the protein and the PDA component. In the dual amplification mode, the first signal comes from capture of the antibody-conjugated PDA-rGO to form sandwiched immunocomplexes on the SPRi chip, followed by a PDA-induced spontaneous gold reductive deposition on PDA-rGO to further enhance the SPRi signal. The detection limit as low as 500 pg mL-1 is achieved on a nonfouling SPRi chip widi high specificity and a wide dynamic range for a model biomarker, carcinoembryonic antigen (CEA) in 10% human serum. [ABSTRACT FROM AUTHOR] |
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| Database: |
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