| Title: |
A Small Molecule Agonist of EphA2 Receptor Tyrosine Kinase Inhibits Tumor Cell Migration In Vitro and Prostate Cancer Metastasis In Vivo. |
| Authors: |
Petty, Aaron1, Myshkin, Eugene1, Haina Qin2, Hong Guo1, Hui Miao1, Tochtrop, Gregory P.3,4, Jer-Tsong Hsieh5, Page, Phillip6, Lili Liu7, Lindner, Daniel J.8, Acharya, Chayan9, MacKerell Jr., Alexander D.9, Ficker, Eckhard1, Jianxing Song2,10 bchsj@nus.edu.sg, Bingcheng Wang1,4,11 bxw14@case.edu, Ming Tat Ling12 |
| Source: |
PLoS ONE. Aug2012, Vol. 7 Issue 8, Special section p1-14. 14p. |
| Subject Terms: |
*CANCER invasiveness, *METASTASIS, *DOXAZOSIN, *LIGANDS (Biochemistry), *CHEMICAL agonists, *PROSTATE cancer |
| Abstract: |
During tumor progression, EphA2 receptor can gain ligand-independent pro- oncogenic functions due to Akt activation and reduced ephrin-A ligand engagement. The effects can be reversed by ligand stimulation, which triggers the intrinsic tumor suppressive signaling pathways of EphA2 including inhibition of PI3/Akt and Ras/ERK pathways. These observations argue for development of small molecule agonists for EphA2 as potential tumor intervention agents. Through virtual screening and cell-based assays, we report here the identification and characterization of doxazosin as a novel small molecule agonist for EphA2 and EphA4, but not for other Eph receptors tested. NMR studies revealed extensive contacts of doxazosin with EphA2/A4, recapitulating both hydrophobic and electrostatic interactions recently found in the EphA2/ephrin-A1 complex. Clinically used as an α1-adrenoreceptor antagonist (Cardura®) for treating hypertension and benign prostate hyperplasia, doxazosin activated EphA2 independent of α1-adrenoreceptor. Similar to ephrin-A1, doxazosin inhibited Akt and ERK kinase activities in an EphA2-dependent manner. Treatment with doxazosin triggered EphA2 receptor internalization, and suppressed haptotactic and chemotactic migration of prostate cancer, breast cancer, and glioma cells. Moreover, in an orthotopic xenograft model, doxazosin reduced distal metastasis of human prostate cancer cells and prolonged survival in recipient mice. To our knowledge, doxazosin is the first small molecule agonist of a receptor tyrosine kinase that is capable of inhibiting malignant behaviors in vitro and in vivo. [ABSTRACT FROM AUTHOR] |
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| Database: |
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