Bibliographic Details
| Title: |
The haemolysin-secreting ShlB protein of the outer membrane of Serratia marcescens : determination of surface-exposed residues and formation of ion-permeable pores by ShlB mutants in artificial lipid bilayer membranes. |
| Authors: |
Könninger, Ulrich W., Hobbie, Silke, Benz, Roland, Braun, Volkmar |
| Source: |
Molecular Microbiology. Jun99, Vol. 32 Issue 6, p1212-1225. 14p. |
| Subject Terms: |
*MICROBIAL proteins, *SERRATIA marcescens, *MOLECULAR microbiology |
| Abstract: |
The ShlB protein in the outer membrane of Serratia marcescens is the only protein known to be involved in secretion of the ShlA protein across the outer membrane. At the same time, ShlB converts ShlA into a haemolytic and a cytolytic toxin. Surface-exposed residues of ShlB were determined by reaction of an M2 monoclonal antibody with the M2 epitope DYKDDDDK inserted at 25 sites along the entire ShlB polypeptide. The antibody bound to the M2 epitope at 17 sites in intact cells, which indicated surface exposure of the epitope, and to 23 sites in isolated outer membranes. Two insertion mutants contained no ShlB(M2) protein in the outer membrane. The ShlB derivatives activated and/or secreted ShlA. To gain insights into the secretion mechanism, we studied whether highly purified ShlB and ShlB deletion derivatives formed pores in artificial lipid bilayer membranes. Wild-type ShlB formed channels with very low single channel conductance that rarely assumed an open channel configuration. In contrast, open channels with a considerably higher single channel conductance were observed with the deletion mutants ShlB(Δ65–186), ShlB(Δ87–153), and ShlB(Δ126–200). ShlB(Δ126–200) frequently formed permanently open channels, whereas the conductance caused by ShlB(Δ65–186) and ShlB(Δ87–153) did not assume a stationary value, but fluctuated rapidly between open and closed configurations. The results demonstrate the orientation of large portions of ShlB in the outer membrane and suggest that ShlB may function as a specialized pore through which ShlA is secreted. [ABSTRACT FROM AUTHOR] |
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