Bibliographic Details
| Title: |
Regulation of -1 ribosomal frameshifting directed by Cocksfoot mottle sobemovirus genome. |
| Authors: |
Lucchesi, Jimmy1, Mäkeläinen, Katri1, Merits, Andres1, Tamm, Tiina2, Mäkinen, Kristiina1 |
| Source: |
European Journal of Biochemistry. Jun2000 Part 2, Vol. 267 Issue 12, p3523-3529. 7p. |
| Subject Terms: |
*PLANT viruses, *RIBOSOMES, *GENOMES |
| Abstract: |
The polyprotein of Cocksfoot mottle virus (CfMV) is encoded by two overlapping open reading frames (ORFs). The putative replicase of CfMV is produced as a part of the polyprotein from ORF2b by the -1 ribosomal frameshifting mechanism. The signals leading to -1 ribosomal frameshifting directed by CfMV RNA are the slippery heptamer UUUAAAC and a stem–loop structure starting seven nucleotides downstream from the heptamer. We studied the effect of different parts of the CfMV genome on the -1 ribosomal frameshifting efficiency using a wheat germ extract transcription/translation system. A point mutation in the slippery heptamer and a mutation deleting the stem–loop structure prevented frameshifting. Seventy nucleotides of CfMV sequence, including the slippery sequence and the stem–loop structure, was found to act as a minimal region for frameshifting. Interestingly, a termination codon introduced into the -1-frame 27 nucleotides downstream of the stem–loop structure increased frameshift efficiency threefold, while a similarly located termination codon in the 0-frame had no effect. Even fourfold to fivefold efficiencies were observed when the polyprotein encoding ORFs were fused together, which led simultaneously to the formation of a termination codon downstream of the frameshift signal. Possible reasons underlying these observations are discussed. [ABSTRACT FROM AUTHOR] |
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| Database: |
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