Bibliographic Details
| Title: |
Molecular cloning of a novel secreted peptide, INM02, and regulation of its expression by glucose. |
| Authors: |
Xuanchun Wang1 |
| Source: |
Journal of Endocrinology. Sep2009, Vol. 202 Issue 3, p355-364. 10p. |
| Subject Terms: |
*MOLECULAR cloning, *PEPTIDES, *GENETIC regulation, *GLUCOSE, *MESSENGER RNA, *AMINO acids, *GENETIC code, *ISLANDS of Langerhans, *ENZYME-linked immunosorbent assay |
| Abstract: |
We report the identification of a novel secreted peptide, INM02. The mRNA transcript of human INM02 gene is about 3.0 kb. Its open-reading frame contains 762 bps and encodes a protein of 254 amino acids. Northern blot analysis demonstrates that INM02 mRNA is widely expressed in rat tissues, especially with abundant quantities in pancreatic islets, testis, and bladder tissue. We have expressed recombinant INM02 protein and generated rabbit anti-INM02 polyclonal antibodies. We show here that INM02 could be detectable in human serum by ELISA. We also present evidence that INM02 mRNA expression could be regulated by glucose. Experiments on both MIN6 cells and intact isolated islets demonstrate that INM02 mRNA levels are increased more than threefold by high glucose (25 mM) when compared with low glucose (5.5 mM). ELISA analysis shows that secretion of INM02 is significantly augmented by high glucose in vitro. It is speculated that as a novel secreted protein, INM02 is associated with functions of pancreatic islets, especially of β-cells. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Endocrinology is the property of Bioscientifica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Complete |
