Bibliographic Details
| Title: |
Direct in vitro Multiple Shoot Regeneration of Local Berseem (Trifolium alexandrinum L.) Cultivars. |
| Authors: |
Anjum, Muhammad Nisar1, Joyia, Faiz Ahmad1 faizahmad1980@gmail.com, Khan, Muhammad Sarwar1, Ali, Muhammad Amjad2 |
| Source: |
Pakistan Journal of Agricultural Sciences. 2025, Vol. 62 Issue 1, p69-77. 9p. |
| Subject Terms: |
*FODDER crops, *GROWTH regulators, *HYPOCOTYLS, *POTTING soils, *CLOVER |
| Abstract: |
Berseem (Trifolium alexandrinum L.) is an important winter fodder crop. The objective of this study was to develop an efficient regeneration protocol applicable to multiple berseem genotypes. To achieve this, four genotypes including (Superlate Faisalabad, Anmol, Agaiti berseem, and SB-10) were selected for direct in vitro regeneration. We have reported an efficient in vitro regeneration protocol which is suitable for local cultivars. We optimized the protocol by utilizing different explants such as intact cotyledons, wounded cotyledons, and hypocotyls. MS regeneration media supplemented with different growth regulators/combinations (Auxin, cytokinin, and gibberellin) at different ratios were used for these explants. The highest shoot regeneration was observed in intact cotyledons on MS Media augmented with NAA/BAP at ratios BAP 2.0 mg/L & NAA 0.05 mg/L compared to wounded cotyledons and hypocotyls of "Superlate Faisalabad" genotype. Individual shoots were separated and transferred to IBA, IAA-containing, or IBA-free MS media for rooting. Plantlets that had regenerated and established good root system were placed in pots filled with garden soil and kept in a greenhouse where they had a 95% survival rate. Establishing such an efficient direct in vitro regeneration protocol paves the way for future genetic modification in Trifolium to incorporate desirable traits. [ABSTRACT FROM AUTHOR] |
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| Database: |
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