Bibliographic Details
| Title: |
Phenotypic and Genomic Assessment of Antimicrobial Resistance and Virulence Factors Determinants in Salmonella Heidelberg Isolated from Broiler Chickens. |
| Authors: |
de Almeida Figueira, Arthur1 (AUTHOR), Salles Dias, Thomas1,2 (AUTHOR) thomassalles@id.uff.br, Alves Costa, Gisllany1 (AUTHOR), Lima da Costa Abreu, Dayse1,2 (AUTHOR), dos Santos Medeiros, Luciana2 (AUTHOR), Léo de Almeida Pereira, Virginia1,2 (AUTHOR) |
| Source: |
Animals (2076-2615). Apr2025, Vol. 15 Issue 7, p1003. 10p. |
| Subject Terms: |
*WHOLE genome sequencing, *FOODBORNE diseases, *POULTRY products, *POULTRY as food, *DRUG resistance in microorganisms |
| Abstract: |
Simple Summary: In our study, we investigated antimicrobial resistance in Salmonella Heidelberg, a bacterium commonly found in poultry and poultry food products. We analyzed 14 strains isolated from chickens in Brazil between 2013 and 2019, testing their phenotypic resistance to various antimicrobials and performing genomic analysis to identify the genes responsible for this resistance. Our results revealed a high frequency of resistance to commonly used antibiotics, such as cephalosporins, tetracyclines, and sulfonamides. All samples carried specific genetic mutations and resistance genes that could enhance their resistance and potentially increase their virulence. Notably, we identified genes typically associated with Yersinia, a pathogen that has been linked to more severe infections, suggesting that these Salmonella strains may pose a greater risk than previously expected. These findings underscore the need for continued surveillance to prevent foodborne illness and ensure the effectiveness of infection treatments. Salmonella Heidelberg is frequently found in poultry and poultry products and is associated with antimicrobial resistance strains and infections and mortality in humans. Whole-genome sequencing is used to monitor and understand epidemiological factors related to antimicrobial resistance. This study aimed to characterize the phenotypic resistance and sequence the whole genome of Salmonella Heidelberg strains isolated from poultry products in Brazil. Fourteen Salmonella Heidelberg strains isolated from whole broiler chicken carcasses and portions in Brazil between 2013 and 2019 were used in this study. Genus confirmation was performed by polymerase chain reaction. The disk diffusion test was conducted to assess the phenotypical antimicrobial susceptibility of the strains. Whole-genome sequencing was carried out to investigate the presence of antimicrobial resistance genes, plasmids, multilocus sequence typing, and virulence-associated genes. A high frequency of phenotypic resistance to cephalosporins, tetracyclines, and sulfonamides was detected. All strains had mutations in gyrA and parC and contained the genes tet(A), fosA7, and sul. The presence of genes originating from Yersinia pathogenicity islands was also detected. This study identified a high frequency of antimicrobial resistance in Salmonella Heidelberg strains from broilers slaughtered in different regions of Brazil, all belonging to the same sequence type (ST15) and associated with multiple resistance and virulence genes. The presence of the Yersinia high-pathogenicity island was detected, indicating potential virulence. These findings highlight the importance of continuously monitoring antimicrobial resistance to control and prevent foodborne infections and maintain the efficacy of treatments for human salmonellosis. [ABSTRACT FROM AUTHOR] |
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