Bibliographic Details
| Title: |
Correlations of Free Thyroid Hormones Measured by Tandem Mass Spectrometry and Immunoassay in Dogs. |
| Authors: |
Sasso, Raffaella1 (AUTHOR) raffaella.sasso@uniba.it, Siniscalchi, Marcello1,2 (AUTHOR), d'Ingeo, Serenella1 (AUTHOR), Ventriglia, Gianluca1,2 (AUTHOR), Bitetto, Candida2 (AUTHOR), Quaranta, Angelo1 (AUTHOR) |
| Source: |
Animals (2076-2615). Mar2025, Vol. 15 Issue 5, p689. 16p. |
| Subject Terms: |
*TANDEM mass spectrometry, *CHEMILUMINESCENCE immunoassay, *THYROID hormones, *ENZYME-linked immunosorbent assay, *MASS spectrometry, *THYROID gland function tests |
| Abstract: |
Simple Summary: The diagnosis of primary hypothyroidism in dogs is confirmed by a reduced serum total thyroxine (TT4) or free thyroxine (FT4) level associated with an elevated serum thyroid-stimulating hormone (TSH) level. To formulate a diagnosis, in addition to a correct evaluation of the medical history, direct physical examination and haemato-biochemical profile, an accurate measurement of the serum concentration of thyroid hormones is important. The aim of this study is to evaluate FT4 in suspected hypothyroid dogs by comparing two of the most accredited assay techniques in common veterinary laboratories: chemiluminescence enzyme immunoassay (CLEIA) and liquid chromatography–tandem mass spectrometry (LC-MS/MS) preceded by ultrafiltration. The results demonstrate that CLEIA, which is more advantageous and practical from an economic point of view, can be used for screening, while LC-MS/MS is recommended for a more precise evaluation of thyroid function in more complex clinical cases such as in dogs with altered thyroid hormone values. A significant and statistical positive correlation was observed between blood FT4 values measured by liquid chromatography–tandem mass spectrometry and chemiluminescence both in subjects with normal FT4 and TSH values and in the group of dogs that presented these altered values. Furthermore, a negative and significant association was observed between blood TSH and FT4 values measured with tandem mass spectrometry in the group of dogs with hormonal values attributable to hypothyroidism. Thyroid function is commonly assessed in dogs through serum measurements of free thyroxine (FT4) and thyroid-stimulating hormone (TSH). The accurate measurement of thyroid hormones is critical. In particular, the measurement of free thyroxine (FT4) is important for the diagnosis and management of thyroid disorders. Most laboratories measure FT4 using direct analog immunoassay methods. The reliability of the results obtained using these methods has often been questioned. Fifty-nine dogs for which the veterinarian had requested the evaluation of thyroid hormones were included in this study. FT4 and TSH were measured using chemiluminescence enzyme immunoassay (CLEIA). Furthermore, FT4 was also measured by liquid chromatography–tandem mass spectrometry (LC-MS/MS) preceded by ultrafiltration. Comparing FT4 with the two different methods, the results demonstrate that LC-MS/MS, preceded by ultrafiltration, provides more accurate and specific FT4 measurements, particularly in dogs with altered thyroid hormone values. Although CLEIA is cost-effective and widely used for screening, LC-MS/MS is recommended for the more precise assessment of thyroid function. [ABSTRACT FROM AUTHOR] |
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