Bibliographic Details
| Title: |
Impact of Low-Temperature Storage on Growth Characteristics of Marine Diatom, Navicula sp. |
| Authors: |
Abishag, M. Muthu1 (AUTHOR) muthuabishag@tnfu.ac.in, Faizullah, M. Mohammed1 (AUTHOR), Balasundari, S.1 (AUTHOR) |
| Source: |
Journal of Coastal Research. 2025, Vol. 41 Issue 2, p214-222. 9p. |
| Subject Terms: |
*DIMETHYL sulfoxide, *FISH hatcheries, *BIOTECHNOLOGY, *NAVICULA, *SHRIMP culture |
| Abstract: |
Abishag, M.M.; Faizullah, M.M., and Balasundari, S., 2025. Impact of low-temperature storage on growth characteristics of marine diatom, Navicula sp. Journal of Coastal Research, 41(2), 214–222. Charlotte (North Carolina), ISSN 0749-0208. In fish and shellfish hatcheries, the traditional methods of microalgal preservation by subculturing are labour intensive and result in decrease of cell size and viability. Hence, long-term preservation without deterioration of genetic and nutritional quality of the commercially important diatom, Navicula sp., can be achieved through low-temperature preservation techniques. This study aimed to assess the growth characteristics of Navicula sp. preserved at different low temperatures to identify the best protocol for preservation. Navicula sp. was isolated from shrimp ponds and subjected to freezing at –4°C, –20°C, and –196°C at two different concentrations of dimethyl sulphoxide (Me2SO; 5% and 10%) and freezing rates (direct plunging and two-step freezing at –196°C) for 30 days. Growth characteristics and viability were assessed postthawing. Concerning the biomass at the exponential phase, cells preserved at –20°C and –196°C with 5% cryoprotectant exhibited comparatively higher biomass than the cells preserved with 10% cryoprotectant at the same temperature (p ≤ 0.05) upon thawing. The highest biomass and cell density were observed in the thawed Navicula sp. preserved at –196°C with 5% Me2SO after the two-step freezing protocol (p ≤ 0.05). However, the cells preserved at higher temperature regimes, at –4°C and –20°C, had higher carotenoid concentrations when compared with cells preserved at –196°C. The cells preserved at –196°C after the two-step freezing protocol were viable for a longer period (12 d) when compared with cells preserved at –20°C (7 d). Thus, considering the growth characteristics, Navicula sp. can be preserved at –196°C with 5% Me2SO after the two-step freezing protocol for long-duration preservation for use as stock culture for feeding in shrimp hatcheries and nurseries and for further biotechnological interventions. [ABSTRACT FROM AUTHOR] |
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