Bibliographic Details
| Title: |
Analysis the diagnostic performance of H4C6/SOX1-OT gene methylation in bladder cancer based on urine sample. |
| Authors: |
Wang, Wan-Dang1,2 (AUTHOR), Yang, Xu-Ran1 (AUTHOR), Li, Wen-Hua1 (AUTHOR), Cheng, Jing-Mao1,2 (AUTHOR), Wu, Jing-Yi1 (AUTHOR), Cai, Jin-Mei1 (AUTHOR), Chen, Hui1 (AUTHOR) tinarun_2007@126.com |
| Source: |
Scientific Reports. 2/26/2025, Vol. 15 Issue 1, p1-9. 9p. |
| Subject Terms: |
*BLADDER cancer, *TUMOR grading, *DNA methylation, *TUMOR classification, *CASE-control method |
| Abstract: |
Bladder cancer (BCa) is the second most common urological malignancy, but the techniques used today to detect and monitor BCa are frequently invasive and/or have inadequate sensitivity and specificity. Therefore, it is imperative to create a noninvasive test that is both sensitive and accurate for diagnosing BCa. This research introduces and validates the diagnostic performance of H4C6/SOX1-OT gene methylation in the diagnosis of BCa based on urine samples by designing two parts of studies: the case-control study and the prospective validation study. In the case-control study, the methylation test of H4C6/SOX1-OT achieved a sensitivity/specificity/positive predictive value (PPV)/negative predictive value (NPV) of 87.9%(95%CI, 79.4%–93.3%)/90.4%(95%CI, 80.7%–95.7%)/92.6%(95%CI, 84.8%–96.7%)/ 84.6%(95%CI, 74.3%–91.5%) (kappa value 77.6%). The sensitivities for low grade, high grade, Ta-T1, and T2-T4 were 85% (17/20), 88.6% (70/79), 85.4% (41/48) and 92.6% (25/27). Statistical analysis showed the diagnostic sensitivity of test was not affected by sex, age, tumor grade or tumor stage (P > 0.05). In the prospective validation study, the H4C6/SOX1-OT methylation test yielded an overall sensitivity/specificity/PPV/NPV of 84.8%(95%CI, 67.3%–94.3%)/90.0%(95%CI, 75.4%–96.7%)/87.5% (95%CI, 70.1%–95.9%)/84.6% (95%CI, 73.0%-95.4%) (kappa value 75.0%), indicating 38.4% of spared cystoscopy. These findings highlight the potential of the H4C6/SOX1-OT methylation in urine DNA as a promising molecular diagnostic tool for detecting BCa, especially for early-stage tumors, which may reduce the need for cystoscopy. [ABSTRACT FROM AUTHOR] |
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