Bibliographic Details
| Title: |
Tracing Staphylococcus capitis and Staphylococcus epidermidis strains causing septicemia in extremely preterm infants to the skin, mouth, and gut microbiota. |
| Authors: |
Nowrouzian, Forough L.1 forough.nowrouzian@microbio.gu.se, Lumingkit, Kirth1, Gio-Batta, Monica1, Jaén-Luchoro, Daniel2, Thordarson, Thordur3,4, Elfvin, Anders3,4, Wold, Agnes E.1, Adlerberth, Ingegerd1 |
| Source: |
Applied & Environmental Microbiology. Jan2025, Vol. 91 Issue 1, p1-20. 20p. |
| Subject Terms: |
*RAPD technique, *ORAL microbiology, *TIME-of-flight mass spectrometry, *WHOLE genome sequencing, *PREMATURE infants |
| Abstract: |
Coagulase-negative staphylococci (CoNS) comprise about 50 species, some of which cause septicemia in preterm neonates. CoNS establish early on the skin and in the oral and gut microbiota, from where they may spread to the bloodstream. The colonization pattern preceding septicemia is not well-defined. Forty-two extremely preterm neonates (≤28 + 0 gestational weeks) were followed from birth to 2 months with regular sampling and culturing of the skin and oral and gut microbiota. Blood samples were drawn upon clinical suspicion of septicemia and cultured. CoNS species were identified using matrix-assisted laser-desorption ionization time of flight mass spectrometry (MALDI-TOF). Random amplified polymorphic DNA was used for strain typing, and strains were characterized regarding biofilm production and virulence gene carriage. CoNS blood isolates underwent whole genome sequencing. Staphylococcus epidermidis represented 72% of the CoNS isolates on skin or mucous membranes, followed by Staphylococcus capitis (13%) and Staphylococcus haemolyticus (7%). CoNS septicemia was diagnosed in nine infants, yielding 11 septicemia isolates: seven S. capitis and four S. epidermidis, of which nine were further analyzed. The S. capitis septicemia isolates belonged to the NRCS-A clone. Two-thirds of the septicemia strains were traced back to the commensal microbiota. Colonization of the oral cavity by S. capitis was significantly associated with CoNS septicemia development, although the blood-borne S. capitis strains were more commonly found on the skin than in the mouth prior to invasion. Biofilm production was not associated with septicemia. Our results implicate CoNS colonization as a step that precedes septicemia in preterm neonates. Early colonization of the oral cavity by S. capitis may represent a particular risk. [ABSTRACT FROM AUTHOR] |
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| Database: |
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