Bibliographic Details
| Title: |
Generation of IgM+ B cell-deficient Atlantic salmon (Salmo salar) by CRISPR/Cas9-mediated IgM knockout. |
| Authors: |
Raudstein, Mari1 (AUTHOR), Peñaranda, Ma. Michelle D.1 (AUTHOR), Kjærner-Semb, Erik1 (AUTHOR), Grove, Søren1 (AUTHOR), Morton, H. Craig1 (AUTHOR), Edvardsen, Rolf Brudvik1 (AUTHOR) rolfbe@hi.no |
| Source: |
Scientific Reports. 1/28/2025, Vol. 15 Issue 1, p1-13. 13p. |
| Subject Terms: |
*MEDICAL sciences, *ATLANTIC salmon, *LIFE sciences, *WESTERN immunoblotting, *SALMON farming, *IMMUNOGLOBULIN M, *B cells |
| Abstract: |
Infectious diseases pose significant challenges to Norwegian Atlantic salmon aquaculture. Vaccines are critical for disease prevention; however, a deeper understanding of the immune system is essential to improve vaccine efficacy. Immunoglobulin M (IgM) is the main antibody involved in the systemic immune response of teleosts, including Atlantic salmon. In this study, we used CRISPR/Cas9 technology to knock out the two IgM genes in Atlantic salmon. High-throughput sequencing revealed an average mutagenesis efficiency of 97% across both loci, with a predominance of frameshift mutations (78%). Gene expression analyses demonstrated significantly reduced membrane-bound IgM mRNA levels in head kidney and spleen tissues. Flow cytometry revealed a 78% reduction in IgM+ B cells in peripheral blood, and Western blot analyses showed decreased IgM protein levels in serum. Notably, an upregulation of IgT mRNA was observed, suggesting a potential compensatory mechanism. This work presents the first application of CRISPR/Cas9 to disrupt an immune-related gene in the F0 generation of Atlantic salmon, and lays the foundation for generating a model completely lacking IgM+ B cells which can be used to study the role of B cells and antibodies. This study has implications for advancing immune research in teleosts and for developing strategies to improve salmon health and welfare in aquaculture. [ABSTRACT FROM AUTHOR] |
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