Bibliographic Details
| Title: |
Generation of Codon-Optimized Fad3 Gene Transgenic Bovine That Produce More n-3 Polyunsaturated Fatty Acids. |
| Authors: |
Su, Guanghua1,2 (AUTHOR) guanghuasu@imu.edu.cn, Wei, Zhuying1,2 (AUTHOR) chunling1980_0@163.com, Bai, Chunling1,2 (AUTHOR) lidanyi0215@163.com, Li, Danyi1,2 (AUTHOR) zhaoxiaoyu3233@163.com, Zhao, Xiaoyu1,2 (AUTHOR) liuxuefei1006@126.com, Liu, Xuefei1,2 (AUTHOR) xiaoshuang2000@126.com, Song, Lishuang1,2 (AUTHOR) zhanglinmg@aliyun.com, Zhang, Li1,2 (AUTHOR) gpengli@imu.edu.cn, Li, Guangpeng1,2 (AUTHOR), Yang, Lei1,2 (AUTHOR) guanghuasu@imu.edu.cn |
| Source: |
Animals (2076-2615). Jan2025, Vol. 15 Issue 1, p93. 15p. |
| Subject Terms: |
*SOMATIC cell nuclear transfer, *FATTY acid desaturase, *OMEGA-6 fatty acids, *OMEGA-3 fatty acids, *CATTLE breeds |
| Abstract: |
Simple Summary: This study successfully created fatty acid desaturase 3 (Fad3) transgenic cattle by utilizing CRISPR-Cas9 technology to insert a codon-optimized Fad3 gene sequence into bovine fibroblast cells and employing somatic cell nuclear transfer (SCNT) technology. Gas chromatographic analysis confirmed that the n-3 PUFA (polyunsaturated fatty acid) content in the transgenic cattle was significantly increased, while the ratio of n-6 PUFAs to n-3 PUFAs decreased. Fad3 transgenic cattle are rich in polyunsaturated fatty acids and represent a high-quality breed of beef cattle. The successful breeding of Fad3 transgenic cattle not only meets the demand for healthy diets but also serves as a model for studying the effects of endogenous n-3 PUFAs on animals. Polyunsaturated fatty acids (PUFAs) such as linoleic acid (18:2, n-6) and α-linolenic acid (18:3, n-3) are essential for the growth, development, and well-being of mammals. However, most mammals, including humans, cannot synthesize n-3 and n-6 PUFAs and these must be obtained through diet. The beneficial effect of converting n-6 polyunsaturated fatty acids (n-6 PUFAs) into n-3 polyunsaturated fatty acids (n-3 PUFAs) has led to extensive research on the flax fatty acid desaturase 3 (Fad3) gene, which encodes fatty acid desaturase. Still, the plant-derived Fad3 gene is used much less in transgenic animals than the Fat-1 gene from Caenorhabditis elegans. To address this problem, we used somatic cell nuclear transfer (SCNT) technology to create codon-optimized Fad3 transgenic cattle. Gas chromatographic analysis showed that the n-3 PUFA content of transgenic cattle increased significantly, and the ratio of n-6 PUFAs to n-3 PUFAs decreased from 3.484 ± 0.46 to about 2.78 ± 0.14 (p < 0.05). In conclusion, Fad3 gene knock-in cattle are expected to improve the nutritional value of beef and can be used as an animal model to study the therapeutic effects of n-3 PUFAs in various diseases. [ABSTRACT FROM AUTHOR] |
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