| Title: |
Interference of flagellar rotation up-regulates the expression of small RNA contributing to Bordetella pertussis infection. |
| Authors: |
Yukihiro Hiramatsu1 yhiramatsu@biken.osaka-u.ac.jp, Takashi Nishida1, Nugraha, Dendi Krisna1, Mayuko Osada-Oka2, Daisuke Nakane3, Katsumi Imada4, Yasuhiko Horiguchi1,5 |
| Source: |
Science Advances. 1/3/2025, Vol. 11 Issue 1, p1-16. 16p. |
| Subject Terms: |
*BORDETELLA pertussis, *GENE expression, *WHOOPING cough, *BACTERIAL RNA, *NON-coding RNA |
| Abstract: |
Bacterial small RNAs (sRNAs) posttranscriptionally regulate gene expressions involved in various biological processes, including pathogenicity. Our previous study identified sRNAs, the expression of which was up-regulated in Bordetella pertussis, the causative agent of whooping cough, upon tracheal colonization of the bacteria; however, their roles in bacterial infection remain unknown. Here, we found that one sRNA, Bpr4, contributes to B. pertussis infection by posttranscriptionally up-regulating filamentous hemagglutinin (FHA), a major adhesin of the bacteria. Bpr4 bound to the 5' untranslated region of fhaB mRNA encoding FHA and inhibited its degradation mediated by RNaseE. Our results demonstrated that Bpr4 up-regulation was triggered by the interference of flagellar rotation, which caused the disengagement of MotA, a flagellar stator. Subsequently, MotA activated a diguanylate cyclase to generate cyclic di-GMP, which plays a role in Bpr4 up-regulation through the RisK/RisA two-component system. Our findings indicate that a flagellum-triggered sensory system contributes to B. pertussis infection. [ABSTRACT FROM AUTHOR] |
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| Database: |
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