Bibliographic Details
| Title: |
Development and Application of a Fully Automated Chemiluminescence Enzyme Immunoassay for the Detection of Antibodies Against Porcine Circovirus 3 Cap. |
| Authors: |
Wang, Lei1,2 (AUTHOR) lduan202412@163.com, Li, Duan2,3,4 (AUTHOR) 19861927085@163.com, Zeng, Daoping3,4 (AUTHOR), Wang, Xiaomin2 (AUTHOR) yll395070943@163.com, Liu, Yanlin2 (AUTHOR), Peng, Guoliang5 (AUTHOR) pengguoliang168@163.com, Xu, Zheng2 (AUTHOR) stonezen@scau.edu.cn, Song, Changxu2 (AUTHOR) stonezen@scau.edu.cn |
| Source: |
Viruses (1999-4915). Dec2024, Vol. 16 Issue 12, p1925. 13p. |
| Subject Terms: |
*CHEMILUMINESCENCE immunoassay, *REFERENCE values, *ENZYME-linked immunosorbent assay, *VIRUS-like particles, *MAGNETIC particles |
| Abstract: |
Porcine circovirus 3 (PCV3) is a small non-enveloped circovirus associated with porcine dermatitis and nephropathy syndrome (PDNS). It has occurred worldwide and poses a serious threat to the pig industry. However, there is no commercially available vaccine. PCV3 capsid protein (Cap) is an ideal antigen candidate for serodiagnosis. Here, a novel fully automated chemiluminescence enzyme immunoassay (CLEIA) was developed to detect antibodies (Abs) to Cap in porcine serum. Recombinant PCV3 Cap, self-assembled into virus-like particles (VLPs), was produced using baculovirus and coupled to magnetic particles (Cap-MPs) as carriers. Combined with an alkaline phosphatase (AP)–adamantane (AMPPD) system, Cap-Abs can be rapidly measured on a fully automated chemiluminescence analyzer. Under optimal conditions, a cut-off value of 31,508 was determined, with a diagnostic sensitivity of 96.8% and specificity of 97.3%. No cross-reactivity was observed with PCV1 and PCV2 and other common porcine pathogens, and both intra-assay and inter-assay coefficients were less than 5% and 10%, respectively. Prepared Cap-MPs can be stored at 4 °C for more than 6 months. Importantly, this CLEIA had a good agreement of 95.19% with the commercially available kit, demonstrating excellent analytical sensitivity and significantly reduced operating time and labor. A serological survey was then conducted, and showed that PCV3 continues to spread widely in South China. In conclusion, our CLEIA provides time and labor-saving, and a reliable tool for PCV3 epidemiological surveillance. [ABSTRACT FROM AUTHOR] |
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