Bibliographic Details
| Title: |
Polarization modulated spectroscopic ellipsometry-based surface plasmon resonance biosensor for E. coli K12 detection. |
| Authors: |
Zangenehzadeh, Soraya1,2 (AUTHOR), Agocs, Emil1,2 (AUTHOR), Schröder, Fenja1 (AUTHOR), Amroun, Nassima1 (AUTHOR), Biedendieck, Rebekka3 (AUTHOR), Jahn, Dieter3 (AUTHOR), Günther, Axel1,2 (AUTHOR), Zheng, Lei2,4 (AUTHOR), Roth, Bernhard2,4 (AUTHOR), Johannes, Hans-Hermann1,2 (AUTHOR) h-h.johannes@tu-braunschweig.de, Kowalsky, Wolfgang1,2 (AUTHOR) |
| Source: |
Scientific Reports. 11/7/2024, Vol. 14 Issue 1, p1-11. 11p. |
| Subject Terms: |
*SURFACE plasmon resonance, *ESCHERICHIA coli, *BORATE crystals, *DETECTION of microorganisms, *DRUG target, *ESCHERICHIA coli O157:H7 |
| Abstract: |
In this work, we report on the application of the polarization modulated spectroscopic ellipsometry-based surface plasmon resonance method for sensitive detection of microorganisms in Kretschmann configuration. So far, rotating analyzer and single wavelength polarization modulation methods have widely been investigated for phase sensitive surface plasmon resonance measurement. In this study, a much simpler optical setup relying on fast electro-optic phase modulator crystals is introduced for bacteria detection. A beta barium borate crystal connected to a function generator is adapted for generating phase shifts in the millisecond regime to extract the ellipsometric angles (Ψ and Δ ) under the surface plasmon resonance condition. For detection, the gold surface was functionalized with anti-Escherichia coli antibodies, and E. coli K12 was attached to them. We show that polarization modulated spectroscopic ellipsometry achieves a refractive index resolution in the order of 10 - 5 RIU, and a limit of detection of 10 2 CFU/mL for E. coli K12 which is compatible with other surface plasmon resonance based phase sensitive methods with more complex detection concepts. As a follow-up step, an optical model can be developed to enhance this biosensor's performance, and applications for sorting and detecting other biological targets will be investigated. [ABSTRACT FROM AUTHOR] |
|
Copyright of Scientific Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Complete |
|
Full text is not displayed to guests. |
Login for full access.
|
