Bibliographic Details
| Title: |
Comparative Effects of Crude Extracts and Bioactive Compounds from Bidens pilosa and Bidens alba on Nonspecific Immune Responses and Antibacterial Activity Against Vibrio sp. in Coculture with Lactic Acid Bacteria in Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) |
| Authors: |
Widodo, Ari1 (AUTHOR) ariwidodo216@gmail.com, Huang, Huai-Ting1 (AUTHOR) twinkleqazwsx784@gmail.com, Dewi, Novi Rosmala1 (AUTHOR) novi.rosmala.d@gmail.com, Chen, Bo-Ying1 (AUTHOR) joey860812@gmail.com, Wu, Yu-Sheng2 (AUTHOR) wuys0313@mail.npust.edu.tw, Hu, Yeh-Fang1 (AUTHOR) yehfanghu@email.ntou.edu.tw, Nan, Fan-Hua1 (AUTHOR) fhnan@mail.ntou.edu.tw |
| Source: |
Animals (2076-2615). Oct2024, Vol. 14 Issue 20, p2990. 23p. |
| Subject Terms: |
*LACTIC acid bacteria, *PATHOGENIC bacteria, *FISH pathogens, *LINOLEIC acid, *HOT water |
| Abstract: |
Simple Summary: Intensive mariculture practices, particularly high stocking densities, have led to suboptimal growth rates and increased susceptibility to diseases in hybrid grouper, a popular species in aquaculture. Therefore, the use of medicinal plants has become promising to increase the immune response and resist disease. We examined the antimicrobial activity against Vibrio sp., because the Vibrio sp. is a common pathogen in fish. This study demonstrated that ethyl caffeate is nontoxic and increases the phagocytic rate, phagocytic index, and respiratory burst of head kidney leukocytes in hybrid grouper. Ethyl caffeate also has potent antimicrobial activity against some pathogenic bacteria in hybrid grouper aquaculture. Ethyl caffeate (EC) is considered a sustainable way to address the current challenges as they are cheap and environmentally friendly. Ultimately, it has potential to increase the metabolite secondary activity of beneficial bacteria. This study investigated the effect of substances on nonspecific immune responses of head kidney leukocytes, the antimicrobial activity against Vibrio sp., as well as the time-kill of Vibrio sp. by combining the substances with lactic acid bacteria (LAB) and Pediococcus sp. The substances are B. pilosa hot water extract, B. pilosa powder extract, B. pilosa methanol extract, B. pilosa ethanol extract, B. alba hot water extract, B. alba powder extract, B. alba methanol extract, B. alba ethanol extract, and bioactive compounds, namely cytopiloyne, flavonoid, phenol, ethyl caffeate, luteolin, chlorogenic acid, butein, and linoleic acid. The results showed that some of them were nontoxic to the head kidney leukocytes, which can increase the phagocytic rate, phagocytic index, and respiratory burst. These substances were able to inhibit the growth of Vibrio sp.; they can even completely kill the pathogenic bacteria. The largest of the inhibition zone formed from the EC group at a concentration range of 5–50 µg/mL against V. parahaemolyticus, V. alginolyticus, and V. harveyi with a value of 19.7 ± 0.56, 19.3 ± 1.53, and 20.6 ± 1.53 mm. Furthermore, the time-kill studies showed that the LAB and P. acidilactici can completely kill the Vibrio sp. at 6 h incubation time, mainly in the group of combination with EC. [ABSTRACT FROM AUTHOR] |
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