Bibliographic Details
| Title: |
Allelic, Genotypic, and Haplotypic Analysis of Cytokine IL17A, IL17F, and Toll-like Receptor TLR4 Gene Polymorphisms in Metabolic-Dysfunction-Associated Steatotic Liver Disease: Insights from an Exploratory Study. |
| Authors: |
Coste, Sorina-Cezara1 (AUTHOR) secara.sorina@umfcluj.ro, Hilda Orășan, Olga1 (AUTHOR) hilda.orasan@umfcluj.ro, Cozma, Angela1 (AUTHOR) angelacozma@umfcluj.ro, Negrean, Vasile1 (AUTHOR) vasile.negrean@umfcluj.ro, Alexescu, Teodora Gabriela1 (AUTHOR) teodora.alexescu@umfcluj.ro, Perne, Mirela Georgiana1 (AUTHOR) alb.mirela@umfcluj.ro, Ciulei, George1 (AUTHOR) george.ciulei@umfcluj.ro, Hangan, Adriana Corina2 (AUTHOR) adriana.hangan@umfcluj.ro, Lucaciu, Roxana Liana3 (AUTHOR) liana.lucaciu@umfcluj.ro, Iancu, Mihaela4 (AUTHOR) miancu@umfcluj.ro, Procopciuc, Lucia-Maria5 (AUTHOR) lprocopciuc@umfcluj.ro |
| Source: |
Life (2075-1729). Oct2024, Vol. 14 Issue 10, p1327. 20p. |
| Subject Terms: |
*INTERLEUKIN-17, *TOLL-like receptors, *GENETIC polymorphisms, *GENOTYPES, *ALLELES |
| Abstract: |
(1) Background: Interleukin 17 (IL17) and toll-like receptor 4 (TLR4) elevate the risk of metabolic and liver diseases. (2) Methods: This study's objective was to explore the association of IL17 and TLR4 gene polymorphisms with MASLD susceptibility and test their effect on serum IL17 and TLR4 levels. A total of 43 patients with MASLD (MASH/MAFL) and 38 healthy individuals were genotyped for IL17F-A7488G, IL17A-G197A, TLR4-Asp299Gly, and TLR4-Thr399Ile polymorphisms using PCR-RFLP. ELISA methods determined IL17F, IL17A, and TLR4 serum levels. (3) Conclusions: Patients carrying the variant genotypes (A/G + G/G) of IL17-A7448G (OR = 5.25), (G/A + A/A) of IL17-G197A (OR = 10.57), (Asp/Gly + Gly/Gly) of TLR4-Asp299Gly (OR = 3.52), or (Thr/Ile + Ile/Ile) of TLR4-Thr399Ile (OR = 9.87) had significantly increased odds of MASH. Genotype (G/A + A/A) of IL17-G197A was significantly associated with the odds of MAFL (p = 0.0166). Allele A of the IL17-G197A polymorphism was significantly related to increased odds of MAFL (OR = 4.13, p = 0.0133). In contrast, allele A of IL17-G197A (OR = 5.41, p = 0.008), allele Gly of TLR4-Asp299Gly (OR = 3.19, p = 0.046), and allele Ile of TLR4-Thr399Ile (OR = 6.94, p = 0.008) polymorphisms were significantly related to an increased risk of MASH. Allele A of IL17A-G197A, allele Gly of TLR4-Asp299Gly, and allele Ile of TLR4-Thr399Ile gene polymorphisms were significantly associated with the increased odds of MASLD. In patients with MASLD, we found significant influence from the IL17A-G197A gene polymorphism on IL17F levels (p = 0.0343). [ABSTRACT FROM AUTHOR] |
|
Copyright of Life (2075-1729) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Complete |
|
Full text is not displayed to guests. |
Login for full access.
|
