| Title: |
Nanoelectrospray based synthesis of large, transportable membranes with integrated membrane proteins. |
| Authors: |
Wilm, Matthias1,2,3,4 (AUTHOR) matthias.wilm@ucd.ie |
| Source: |
Scientific Reports. 10/24/2024, Vol. 14 Issue 1, p1-13. 13p. |
| Subject Terms: |
*BILAYER lipid membranes, *MEMBRANE proteins, *BIOLOGICAL membranes, *LIQUID surfaces, *BUFFER solutions |
| Abstract: |
Membrane proteins tend to be difficult to study since they need to be integrated into a lipid bilayer membrane to function properly. This study presents a method to synthesize a macroscopically large and freely transportable membrane with integrated membrane proteins which is useful for studying membrane proteins and protein complexes in isolation. The method could serve as a blueprint for the production of larger quantities of functionalised membranes for integration into technical devices similar to the MinION DNA sequencer. It is possible to self-assemble larger biological membranes on solid surfaces. However, they cannot be removed from their solid support without destroying them. In transportable form, self-assembled membranes are limited to sizes of about 17 nm in nanodiscs. Here we electrospray a series of molecular layers onto the liquid surface of a buffer solution which creates a flat, liquid environment on the surface that directs the self-assembly of the membrane. This method enables us to experimentally control the membrane composition and to succeed in producing large membranes with integrated OmpG, a transmembrane pore protein. The technique is compatible with the assembly of membrane based protein complexes. Listeriolysin O and pneumolysin efficiently assemble into non-covalent membrane pore complexes of approximately 30 units or more within the surface layer. [ABSTRACT FROM AUTHOR] |
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