Bibliographic Details
| Title: |
Hydrolysis of phospholipid monolayers by phospholipase A2 revealed by heterodyne-detected sum frequency generation (HD-SFG) spectroscopy. |
| Authors: |
Inoue, Ken-ichi1 (AUTHOR) ken-ichi.inoue@tohoku.ac.jp, Yamamoto, Takashi1 (AUTHOR), Hatori, Yosuke1 (AUTHOR), Hiraide, Takeru1 (AUTHOR), Ye, Shen1 (AUTHOR) ye.shen@tohoku.ac.jp |
| Source: |
Journal of Chemical Physics. 10/21/2024, Vol. 161 Issue 15, p1-7. 7p. |
| Subject Terms: |
*PHOTON upconversion, *MONOMOLECULAR films, *AQUEOUS solutions, *FATTY acids, *HYDROLYSIS |
| Abstract: |
Phospholipase A2 (PLA2) catalyzes the hydrolysis of the sn-2 acyl ester linkage in phospholipid, producing lysophospholipid and fatty acid in the presence of Ca2+. The hydrolysis mediated by PLA2 has attracted much interest in various fields, such as pharmacy and biotechnology. It is recognized that PLA2 cannot hydrolyze phospholipid monolayers at high surface coverage. However, the origin of different PLA2 activities is not fully understood yet. The present study investigated the interaction between DPPC (16:0 PC) monolayer and PLA2 using heterodyne-detected sum frequency generation spectroscopy, which is interface-specific spectroscopy and highly sensitive to molecular symmetry based on a second-order nonlinear optical process. It was revealed that PLA2 adsorbs to the DPPC monolayer on the aqueous solution surface only when the surface coverage is low. The adsorption at the low surface coverage significantly changes the interfacial structures of PLA2 and the hydration, which are stabilized by the presence of Ca2+. Therefore, the restriction of the hydrolysis of phospholipid monolayers at high surface coverage can be rationalized by the inhabitation of the PLA2 adsorption. The present study deepens our molecular-level understanding of the hydrolysis of phospholipids by PLA2. [ABSTRACT FROM AUTHOR] |
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| Database: |
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