Bibliographic Details
| Title: |
Human epithelial lung cell toxicity assessment of collected graphite particles from an iron casting industry (in vitro study). |
| Authors: |
Zendehdel, Rezvan1, Hahad, Omar2,3, Panjali, Zahra4 z.panjali@iautmu.ac.ir |
| Source: |
International Journal of Environmental Health Research. Sep2024, Vol. 34 Issue 9, p3126-3135. 10p. |
| Subject Terms: |
*LUNG analysis, *EPITHELIAL cells, *IN vitro studies, *PHARMACOLOGY, *LIGHT, *RESEARCH funding, *CARBON, *ELECTRON microscopy, *OXIDATIVE stress, *DESCRIPTIVE statistics, *GENE expression, *MANUFACTURING industries, *OCCUPATIONAL exposure, *MINERALS, *PARTICULATE matter, *CELL survival, *BIOMARKERS, *TOXICITY testing |
| Geographic Terms: |
IRAN |
| Abstract: |
Workers in the iron casting industries are exposed to various chemicals, especially graphite in furnace process. This study aims to investigate the toxic effects of graphite particles on human lung cells. Particle characteristics were confirmed by electron microscope and light scattering. Cell viability and oxidative stress markers were measured. The expression of oxidative repair genes, namely OGG1, MTH1, and ITPA, was evaluated. The average particle size was determined to be 172.1 ± 11.96 nm. The median inhibition concentration (IC50) of graphite particles was 46.75 µg/mL. Notably, 25 and 50 µg/mL concentrations resulted in significant GSH depletion and MDA production. The high concentration of graphite particles (200 µg/mL) led to OGG1 suppression and increased MTH1 expression. Based on these findings, graphite exposure may induce toxicity in human lung cells by increasing oxidative stress. Further research is necessary to fully understand the mechanisms underlying graphite toxicity. [ABSTRACT FROM AUTHOR] |
|
Copyright of International Journal of Environmental Health Research is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Complete |
|
Full text is not displayed to guests. |
Login for full access.
|
