Bibliographic Details
| Title: |
The Characterization of a Novel PrMADS11 Transcription Factor from Pinus radiata Induced Early in Bent Pine Stem. |
| Authors: |
Méndez, Tamara1 (AUTHOR) tamendez@utalca.cl, Guajardo, Joselin1 (AUTHOR) joguajardo@utalca.cl, Cruz, Nicolás2 (AUTHOR) nicolascruz83@gmail.com, Gutiérrez, Rodrigo A.3 (AUTHOR) rgutierrez@bio.puc.cl, Norambuena, Lorena4 (AUTHOR) lnorambuena@uchile.cl, Vega, Andrea5 (AUTHOR) andrea.vega@uai.cl, Moya-León, María A.1 (AUTHOR) alemoya@utalca.cl, Herrera, Raúl1 (AUTHOR) raherre@utalca.cl |
| Source: |
International Journal of Molecular Sciences. Jul2024, Vol. 25 Issue 13, p7245. 17p. |
| Subject Terms: |
*TRANSCRIPTION factors, *PINUS radiata, *GENE expression, *PINE, *PROTEIN structure |
| Abstract: |
A novel MADS-box transcription factor from Pinus radiata D. Don was characterized. PrMADS11 encodes a protein of 165 amino acids for a MADS-box transcription factor belonging to group II, related to the MIKC protein structure. PrMADS11 was differentially expressed in the stems of pine trees in response to 45° inclination at early times (1 h). Arabidopsis thaliana was stably transformed with a 35S::PrMADS11 construct in an effort to identify the putative targets of PrMADS11. A massive transcriptome analysis revealed 947 differentially expressed genes: 498 genes were up-regulated, and 449 genes were down-regulated due to the over-expression of PrMADS11. The gene ontology analysis highlighted a cell wall remodeling function among the differentially expressed genes, suggesting the active participation of cell wall modification required during the response to vertical stem loss. In addition, the phenylpropanoid pathway was also indicated as a PrMADS11 target, displaying a marked increment in the expression of the genes driven to the biosynthesis of monolignols. The EMSA assays confirmed that PrMADS11 interacts with CArG-box sequences. This TF modulates the gene expression of several molecular pathways, including other TFs, as well as the genes involved in cell wall remodeling. The increment in the lignin content and the genes involved in cell wall dynamics could be an indication of the key role of PrMADS11 in the response to trunk inclination. [ABSTRACT FROM AUTHOR] |
|
Copyright of International Journal of Molecular Sciences is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Complete |
|
Full text is not displayed to guests. |
Login for full access.
|
