| Title: |
Gene Expression Profile of Benign, Intermediate, and Malignant Spitz and Spitzoid Melanocytic Lesions. |
| Authors: |
Giubellino, Alessio1,2 (AUTHOR) agiubell@umn.edu, He, Yuyu1,2 (AUTHOR) zhou1237@umn.edu, Munro, Sarah A.3 (AUTHOR) smunro@umn.edu, Zhou, Yan1 (AUTHOR) songx047@umn.edu, Song, Kyu Young1,2 (AUTHOR) nels2055@umn.edu, Plaza, Jose A.4 (AUTHOR) josea.plaza@osumc.edu, Torres-Cabala, Carlos A.5 (AUTHOR) ctcabala@mdanderson.org, Nelson, Andrew C.1,2 (AUTHOR) |
| Source: |
Cancers. May2024, Vol. 16 Issue 10, p1798. 13p. |
| Subject Terms: |
*RNA metabolism, *MELANOMA diagnosis, *SKIN tumors, *MELANOMA, *RESEARCH funding, *NEVUS, *TUMOR grading, *IMMUNOHISTOCHEMISTRY, *GENE expression profiling, *TUMOR classification, *MOLECULAR pathology, *MOLECULAR diagnosis, *SEQUENCE analysis |
| Abstract: |
Simple Summary: The interpretation of Spitz and Spitzoid melanocytic lesions can be challenging, even among experts in the field. The discovery of genomic aberrations has contributed to better defining these lesions but knowledge gaps remain. Here, we present gene expression analysis of RNA sequencing data as an additional molecular tool to contribute to filling these gaps and better classifying these lesions. By gene expression profiling, we were able to identify distinct categories, suggesting that the use of this tool may help to improve the characterization of these lesions. Spitz and Spitzoid lesions represent one of the most challenging melanocytic neoplasms in dermatopathology. Nosologic classification has been more recently improved by the discovery of novel molecular drivers, particularly translocations. In the current study, we aimed to use an unbiased approach to explore the gene expression profile of a group of melanocytic Spitz and Spitzoid melanocytic lesions ranging from benign lesions to melanoma, including intermediate lesions such as SPARK nevi and atypical Spitz tumors/melanocytomas. Using unsupervised analysis of gene expression data, we found some distinct hierarchical clusters of lesions, including groups characterized by ALK and NTRK translocations. Few non-ALK translocated tumors demonstrated increased ALK expression, confirmed by immunohistochemistry. Spitz tumors with overlapping features of dysplastic nevi, so-called SPARK nevi, appear to have a common gene expression profile by hierarchical clustering. Finally, weighted gene correlation network analysis identified gene modules variably regulated in subtypes of these cases. Thus, gene expression profiling of Spitz and Spitzoid lesions represents a viable instrument for the characterization of these lesions. [ABSTRACT FROM AUTHOR] |
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