Field application of de novo transcriptomic analysis to evaluate the effects of sublethal freshwater salinization on Gasterosteus aculeatus in urban streams.
| Title: | Field application of de novo transcriptomic analysis to evaluate the effects of sublethal freshwater salinization on Gasterosteus aculeatus in urban streams. |
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| Authors: | Escobar-Sierra, Camilo1 (AUTHOR) cescobar@uni-koeln.de, Lampert, Kathrin P.1 (AUTHOR) |
| Source: | PLoS ONE. 3/13/2024, Vol. 19 Issue 3, p1-24. 24p. |
| Subject Terms: | *THREESPINE stickleback, *SALINIZATION, *ATP-binding cassette transporters, *CHLORIDE channels, *FRESH water |
| Geographic Terms: | GERMANY |
| Abstract: | Freshwater salinization poses global challenges for aquatic organisms inhabiting urban streams, impacting their physiology and ecology. However, current salinization research predominantly focuses on mortality endpoints in limited model species, overlooking the sublethal effects on a broader spectrum of organisms and the exploration of adaptive mechanisms and pathways under natural field conditions. To address these gaps, we conducted high-throughput sequencing transcriptomic analysis on the gill tissue of the euryhaline fish Gasterosteus aculeatus, investigating its molecular response to salinity stress in the highly urbanized river Boye, Germany. We found that in stream sections with sublethal concentrations of chloride costly osmoregulatory systems were activated, evidenced by the differential expression of genes related to osmoregulation. Our enrichment analysis revealed differentially expressed genes (DEGs) related to transmembrane transport and regulation of transport and other osmoregulation pathways, which aligns with the crucial role of these pathways in maintaining biological homeostasis. Notably, we identified candidate genes involved in increased osmoregulatory activity under salinity stress, including those responsible for moving ions across membranes: ion channels, ion pumps, and ion transporters. Particularly, genes from the solute carrier family SLC, aquaporin AQP1, chloride channel CLC7, ATP-binding cassette transporter ABCE1, and ATPases member ATAD2 exhibited prominent differential expression. These findings provide insights into the potential molecular mechanisms underlying the adaptive response of euryhaline fish to salinity stress and have implications for their conservation and management in the face of freshwater salinization. [ABSTRACT FROM AUTHOR] |
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| Items | – Name: Title Label: Title Group: Ti Data: Field application of de novo transcriptomic analysis to evaluate the effects of sublethal freshwater salinization on Gasterosteus aculeatus in urban streams. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Escobar-Sierra%2C+Camilo%22">Escobar-Sierra, Camilo</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> cescobar@uni-koeln.de</i><br /><searchLink fieldCode="AR" term="%22Lampert%2C+Kathrin+P%2E%22">Lampert, Kathrin P.</searchLink><relatesTo>1</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22PLoS+ONE%22">PLoS ONE</searchLink>. 3/13/2024, Vol. 19 Issue 3, p1-24. 24p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22THREESPINE+stickleback%22">THREESPINE stickleback</searchLink><br />*<searchLink fieldCode="DE" term="%22SALINIZATION%22">SALINIZATION</searchLink><br />*<searchLink fieldCode="DE" term="%22ATP-binding+cassette+transporters%22">ATP-binding cassette transporters</searchLink><br />*<searchLink fieldCode="DE" term="%22CHLORIDE+channels%22">CHLORIDE channels</searchLink><br />*<searchLink fieldCode="DE" term="%22FRESH+water%22">FRESH water</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22GERMANY%22">GERMANY</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Freshwater salinization poses global challenges for aquatic organisms inhabiting urban streams, impacting their physiology and ecology. However, current salinization research predominantly focuses on mortality endpoints in limited model species, overlooking the sublethal effects on a broader spectrum of organisms and the exploration of adaptive mechanisms and pathways under natural field conditions. To address these gaps, we conducted high-throughput sequencing transcriptomic analysis on the gill tissue of the euryhaline fish Gasterosteus aculeatus, investigating its molecular response to salinity stress in the highly urbanized river Boye, Germany. We found that in stream sections with sublethal concentrations of chloride costly osmoregulatory systems were activated, evidenced by the differential expression of genes related to osmoregulation. Our enrichment analysis revealed differentially expressed genes (DEGs) related to transmembrane transport and regulation of transport and other osmoregulation pathways, which aligns with the crucial role of these pathways in maintaining biological homeostasis. Notably, we identified candidate genes involved in increased osmoregulatory activity under salinity stress, including those responsible for moving ions across membranes: ion channels, ion pumps, and ion transporters. Particularly, genes from the solute carrier family SLC, aquaporin AQP1, chloride channel CLC7, ATP-binding cassette transporter ABCE1, and ATPases member ATAD2 exhibited prominent differential expression. These findings provide insights into the potential molecular mechanisms underlying the adaptive response of euryhaline fish to salinity stress and have implications for their conservation and management in the face of freshwater salinization. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1371/journal.pone.0298213 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 24 StartPage: 1 Subjects: – SubjectFull: GERMANY Type: general – SubjectFull: THREESPINE stickleback Type: general – SubjectFull: SALINIZATION Type: general – SubjectFull: ATP-binding cassette transporters Type: general – SubjectFull: CHLORIDE channels Type: general – SubjectFull: FRESH water Type: general Titles: – TitleFull: Field application of de novo transcriptomic analysis to evaluate the effects of sublethal freshwater salinization on Gasterosteus aculeatus in urban streams. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Escobar-Sierra, Camilo – PersonEntity: Name: NameFull: Lampert, Kathrin P. IsPartOfRelationships: – BibEntity: Dates: – D: 13 M: 03 Text: 3/13/2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 19326203 Numbering: – Type: volume Value: 19 – Type: issue Value: 3 Titles: – TitleFull: PLoS ONE Type: main |
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