Bibliographic Details
| Title: |
In Response: Getting the measure of hepatitis A virus (HAV): A better understanding of the RNA content of HAV reference material and blood donations. |
| Authors: |
Baylis, Sally A.1 (AUTHOR) sally.baylis@pei.de, Chudy, Michael1 (AUTHOR), Blümel, Johannes1 (AUTHOR) |
| Source: |
Transfusion. Feb2024, Vol. 64 Issue 2, p416-418. 3p. |
| Subject Terms: |
*HEPATITIS A virus, *HEPATITIS viruses, *VIRAL hepatitis, *REFERENCE sources, *RNA |
| Abstract: |
This article discusses the characterization of hepatitis A virus (HAV) RNA-positive blood donations during an epidemic in Poland. The study used two nucleic acid amplification technique (NAT)-based methods to screen the donations for HAV RNA. The sensitivity of the two methods was found to be very similar and extremely sensitive. The article also discusses the evaluation of the 1st World Health Organization (WHO) International Standard (IS) for HAV RNA and the use of RT-droplet digital PCR (RT-ddPCR) to determine the HAV RNA content of the IS. The study found that the IU:copy number ratio of the IS was significantly higher than previously reported, suggesting limitations in the sensitivities of the methods used to determine the potency of the IS. The article concludes by highlighting the importance of understanding the HAV RNA load in transfusion transmission and the need for further research in this area. The study was supported by funding from the German Ministry of Health and the authors declare no conflicts of interest. [Extracted from the article] |
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| Database: |
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