Bibliographic Details
| Title: |
Improved environmental detection of Neoparamoeba perurans using sensitive RNA‐based qPCR. |
| Authors: |
Wynne, James W.1 (AUTHOR) james.wynne@csiro.au, Rigby, Megan L.1 (AUTHOR), Maynard, Ben T.1 (AUTHOR), Taylor, Richard S.1 (AUTHOR) |
| Source: |
Journal of Fish Diseases. Feb2024, Vol. 47 Issue 2, p1-5. 5p. |
| Subject Terms: |
*GENETIC variation, *COMPLEMENTARY DNA, *RIBOSOMAL RNA, *ANTISENSE DNA |
| Abstract: |
This article discusses a study on the detection of Neoparamoeba perurans, a parasite that causes amoebic gill disease (AGD) in salmon. The study compares the use of DNA-based and RNA-based assays for detecting N. perurans in seawater samples. The researchers found that the RNA-based assay was more sensitive and reliable than the DNA-based assay, detecting N. perurans even at low concentrations. The study suggests that targeting intracellular RNA may provide a more accurate measure of amoeba abundance. Further research is needed to determine if RNA-based quantification of N. perurans can be used as an early indicator of AGD. [Extracted from the article] |
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| Database: |
Academic Search Complete |
