| Title: |
Green Synthesis and Characterization of Silver Nanoparticles Using Azadirachta indica Seeds Extract: In Vitro and In Vivo Evaluation of Anti-Diabetic Activity. |
| Authors: |
Rehman, Gauhar1 (AUTHOR) gauhar@awkum.edu.pk, Umar, Muhammad1 (AUTHOR) drabid@awkum.edu.pk, Shah, Nasrullah2 (AUTHOR) nasrullah@awkum.edu.pk, Hamayun, Muhammad3 (AUTHOR) hamayun@awkum.edu.pk, Ali, Abid1 (AUTHOR) arifkhanpak83@gmail.com, Khan, Waliullah2 (AUTHOR) drwali@awkum.edu.pk, Khan, Arif1 (AUTHOR) sajjadmohmand891@gmail.com, Ahmad, Sajjad1 (AUTHOR), Alrefaei, Abdulwahed Fahad4 (AUTHOR) afrefaei@ksu.edu.sa, Almutairi, Mikhlid H.4 (AUTHOR) malmutari@ksu.edu.sa, Moon, Yong-Sun5 (AUTHOR) hangulmys@ynu.ac.kr, Ali, Sajid5 (AUTHOR) gauhar@awkum.edu.pk |
| Source: |
Pharmaceuticals (14248247). Dec2023, Vol. 16 Issue 12, p1677. 20p. |
| Subject Terms: |
*NEEM, *FOURIER transform infrared spectrophotometers, *SILVER nanoparticles, *BLOOD sugar, *STREPTOZOTOCIN |
| Abstract: |
Background: Diabetes mellitus (DM) is a non-communicable, life-threatening syndrome that is present all over the world. The use of eco-friendly, cost-effective, and green-synthesised nanoparticles as a medicinal therapy in the treatment of DM is an attractive option. Objective: In the present study, silver nanoparticles (AI-AgNPs) were biosynthesized through the green synthesis method using Azadirachta indica seed extract to evaluate their anti-diabetic potentials. Methods: These nanoparticles were characterized by using UV-visible spectroscopy, Fourier transform infrared spectrophotometers (FTIR), scanning electron microscopy (SEM), DLS, and X-ray diffraction (XRD). The biosynthesized AI-AgNPs and crude extracts of Azadirachta indica seeds were evaluated for anti-diabetic potentials using glucose adsorption assays, glucose uptake by yeast cells assays, and alpha-amylase inhibitory assays. Results: Al-AgNPs showed the highest activity (75 ± 1.528%), while crude extract showed (63 ± 2.5%) glucose uptake by yeast at 80 µg/mL. In the glucose adsorption assay, the highest activity of Al-AgNPs was 10.65 ± 1.58%, while crude extract showed 8.32 ± 0.258% at 30 mM, whereas in the alpha-amylase assay, Al-AgNPs exhibited the maximum activity of 73.85 ± 1.114% and crude extract 65.85 ± 2.101% at 100 µg/mL. The assay results of AI-AgNPs and crude showed substantial dose-dependent activities. Further, anti-diabetic potentials were also investigated in streptozotocin-induced diabetic mice. Mice were administered with AI-AgNPs (10 to 40 mg/kg b.w) for 30 days. Conclusions: The results showed a considerable drop in blood sugar levels, including pancreatic and liver cell regeneration, demonstrating that AI-AgNPs have strong anti-diabetic potential. [ABSTRACT FROM AUTHOR] |
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