Bibliographic Details
| Title: |
A rotationally-driven dynamic solid phase sodium bisulfite conversion disc for forensic epigenetic sample preparation. |
| Authors: |
Turiello, R.1 (AUTHOR) rat3a@virginia.edu, Nouwairi, R. L.1 (AUTHOR), Keller, J.1 (AUTHOR), Cunha, L. L.1 (AUTHOR), Dignan, L. M.1 (AUTHOR), Landers, J. P.1,2,3 (AUTHOR) |
| Source: |
Lab on a Chip. 1/7/2024, p97-112. 16p. |
| Subject Terms: |
*SODIUM bisulfite, *EPIGENETICS, *MICROSATELLITE repeats, *REFERENCE sources, *DATABASES, *TANDEM repeats |
| Abstract: |
The approaches to forensic human identification (HID) are largely comparative in nature, relying upon the comparison of short tandem repeat profiles to known reference materials and/or database profiles. However, many profiles are generated from evidence materials that either do not have a reference material for comparison or do not produce a database hit. As an alternative to individualizing analysis for HID, researchers of forensic DNA have demonstrated that the human epigenome can provide a wealth of information. However, epigenetic analysis requires sodium b_;is_;ulfite c_;onversion (BSC), a sample preparation method that is time-consuming, labor-intensive, prone to contamination, and characterized by DNA loss and fragmentation. To provide an alternative method for BSC that is more amenable to integration with the forensic DNA workflow, we describe a rotationally-driven, microfluidic method for dynamic solid phase-BSC (dSP-BSC) that streamlines the sample preparation process in an automated format, capable of preparing up to four samples in parallel. The method permitted decreased incubation intervals by ∼36% and was assessed for relative DNA recovery and conversion efficiency and compared to gold-standard and enzymatic approaches. [ABSTRACT FROM AUTHOR] |
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| Database: |
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