| Title: |
Multiple Immune Factors Are Involved in Controlling Acute and Chronic Chikungunya Virus Infection. |
| Authors: |
Poo, Yee Suan1,2 (AUTHOR), Rudd, Penny A.1,2 (AUTHOR), Gardner, Joy1 (AUTHOR), Wilson, Jane A. C.1,2 (AUTHOR), Larcher, Thibaut3 (AUTHOR), Colle, Marie-Anne3 (AUTHOR), Le, Thuy T.1 (AUTHOR), Nakaya, Helder I.4 (AUTHOR), Warrilow, David5 (AUTHOR), Allcock, Richard6 (AUTHOR), Bielefeldt-Ohmann, Helle7 (AUTHOR), Schroder, Wayne A.1 (AUTHOR), Khromykh, Alexander A.2 (AUTHOR), Lopez, José A.1,8 (AUTHOR), Suhrbier, Andreas1,2,8 (AUTHOR) Andreas.Suhrbier@qimrberghofer.edu.au |
| Source: |
PLoS Neglected Tropical Diseases. 12/4/2014, Vol. 8 Issue 12, p1-15. 15p. |
| Subject Terms: |
*CHIKUNGUNYA virus, *ALPHAVIRUS diseases, *VIRUS diseases, *ALPHAVIRUSES, *KILLER cells, *T cells, *TYPE I interferons |
| Geographic Terms: |
PAPUA New Guinea, CARIBBEAN |
| Abstract: |
The recent epidemic of the arthritogenic alphavirus, chikungunya virus (CHIKV) has prompted a quest to understand the correlates of protection against virus and disease in order to inform development of new interventions. Herein we highlight the propensity of CHIKV infections to persist long term, both as persistent, steady-state, viraemias in multiple B cell deficient mouse strains, and as persistent RNA (including negative-strand RNA) in wild-type mice. The knockout mouse studies provided evidence for a role for T cells (but not NK cells) in viraemia suppression, and confirmed the role of T cells in arthritis promotion, with vaccine-induced T cells also shown to be arthritogenic in the absence of antibody responses. However, MHC class II-restricted T cells were not required for production of anti-viral IgG2c responses post CHIKV infection. The anti-viral cytokines, TNF and IFNγ, were persistently elevated in persistently infected B and T cell deficient mice, with adoptive transfer of anti-CHIKV antibodies unable to clear permanently the viraemia from these, or B cell deficient, mice. The NOD background increased viraemia and promoted arthritis, with B, T and NK deficient NOD mice showing high-levels of persistent viraemia and ultimately succumbing to encephalitic disease. In wild-type mice persistent CHIKV RNA and negative strand RNA (detected for up to 100 days post infection) was associated with persistence of cellular infiltrates, CHIKV antigen and stimulation of IFNα/β and T cell responses. These studies highlight that, secondary to antibodies, several factors are involved in virus control, and suggest that chronic arthritic disease is a consequence of persistent, replicating and transcriptionally active CHIKV RNA. Author Summary: The largest epidemic ever recorded for chikungunya virus (CHIKV) started in 2004 in Africa, then spread across Asia and recently caused tens of thousands of cases in Papua New Guinea and the Caribbean. This mosquito-borne alphavirus primarily causes an often debilitating, acute and chronic polyarthritis/polyarthalgia. Despite robust anti-viral immune responses CHIKV is able to persist, with such persistence poorly understood and the likely cause of chronic disease. Herein we highlight the propensity of CHIKV to persist long term, both as a persistent viraemia in different B cell deficient mouse strains, but also as persistent viral RNA in wild-type mice. These studies suggest that, aside from antibodies, other immune factors, such as CD4 T cells and TNF, are active in viraemia control. The work also supports the notion that CHIKV disease, with the exception of encephalitis, is largely an immunopathology. Persistent CHIKV RNA in wild-type mice continues to stimulate type I interferon and T cell responses, with this model of chronic disease recapitulating many of the features seen in chronic CHIKV patients. [ABSTRACT FROM AUTHOR] |
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