Bibliographic Details
| Title: |
The Anti-cancer Effect of Two Extract Fractions from the Hemiscorpius lepturus Scorpion Venom. |
| Authors: |
Setayesh-Mehr, Z.1 (AUTHOR) setayeshmehr@uoz.ac.ir, Asoodeh, A.2 (AUTHOR), Ghasemi, L. V.1 (AUTHOR) |
| Source: |
Applied Biochemistry & Microbiology. Dec2023, Vol. 59 Issue 6, p850-857. 8p. |
| Subject Terms: |
*SCORPION venom, *VENOM, *ANTINEOPLASTIC agents, *ERYTHROCYTES, *P53 antioncogene, *GENE expression |
| Abstract: |
In this study, the fraction of the Hemiscorpius lepturus scorpion venom was subjected. The two bioactive fractions (F2 and F4) were separated and collected by RP-HPLC. Next, the cytotoxicity of F2 and F4 against human red blood cells and MCF-7 cell line was investigated. The expression levels of Bax, Bcl-2, and p53 genes in cancer cells treated with fractions were determined. In order to evaluate the effects of the two extract fractions, breast cancer was induced in BALB-c mice developed by the injection of MCF-7 cells. Following the intraperitoneal injection of F2 and F4 into mice, the levels of IFN-γ and IL-4 were measured using ELISA. The effects of fractions on the survival of MCF-7 cancer cells were significant compared to normal cells (p < 0.001), while they had no significant hemolysis activity against red blood cells (p > 0.05). The results showed that the expression of Bax and p53 was increased in MCF-7 cells treated with fractions compared to control cells without treatment, while the expression of Bcl-2 was decreased. The results of the in vivo analysis showed that the level of IFN-γ was increased, whereas the level of IL-4 was significantly reduced in mice treated with F2 and F4 at the concentrations of 5 and 10 mg/kg compared to control animals (p < 0.0001). Based on the results, two extract fractions of H. lepturus scorpion venom exert anti-cancer properties, and they are able to modulate the immune system and induce apoptosis in cancer cells. [ABSTRACT FROM AUTHOR] |
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