Bibliographic Details
| Title: |
Isolation and Identification of Acetobacter tropicalis From Selected Malaysian Local Fruits for Potential BC Production. |
| Authors: |
Tan Yong Jie1, Zakaria, Junaidi2 junaidibz@umpsa.edu.my, Mohamad, Shahril2, Chua Gek Kee2, Latif, Nurshahqifah1, Ab Rahim, Mohd Hairul1 mhairul@umpsa.edu.my |
| Source: |
Malaysian Applied Biology. Oct2023, Vol. 52 Issue 4, p133-143. 11p. |
| Subject Terms: |
*ACETOBACTER, *ACETOBACTER xylinum, *FRUIT, *BROWN sugar, *JACKFRUIT, *PINEAPPLE, *BANANAS |
| Abstract: |
Acetobacter spp. that are commonly found on fruits, can perform oxidation processes, resulting in acetic acid production in vinegar. Besides that, Acetobacter spp. able to produce bacterial cellulose (BC), which is an essential by-product. This present study was carried out to isolate Acetobacter spp. from selected local fruits. Species verification of the bacterial isolates was performed using molecular and bioinformatic approaches. A total of six local fruits (starfruit, jackfruit, watermelon, pineapple, honeydew & banana) were subjected to seven days of fermentation in a brown sugar solution. Acetobacter spp. were isolated from the fermented medium using bromocresol green ethanol agar as the selective medium. Thirteen bacterial isolates were obtained and subjected to molecular works, including DNA extraction and PCR amplification using universal primers, targeting the 16S rRNA genes. PCRamplified products were selected for single-pass sequencing. BLASTn analysis of the sequencing results showed three isolates (23.1%) belonging to Acetobacter tropicalis and one isolate (7.7%) representing Gluconobacter oxydans might have potential in BC production. However, the remaining nine isolates (69.2%) hit the Lactobacillus genus. Morphological observation using FESEM showed that the BC produced by all the positive bacterial isolates is similar to dried nata de coco and BC produced by Acetobacter xylinum. In addition, four similar regions of -OH stretch (3400 - 3300 cm-1), -CH stretch (2970 to 2800 cm-1), -OH bending (1620 cm-1), and -COC stretch (1100 to 1073 cm-1) are identified in the BC samples. In the future, the isolated Acetobacter and Gluconobacter strains could be further utilized for large-scale BC production in a suitable fermentation medium. [ABSTRACT FROM AUTHOR] |
|
Copyright of Malaysian Applied Biology is the property of Malaysian Society of Applied Biology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Complete |
