Bibliographic Details
| Title: |
A KASP Marker for the Potato Late Blight Resistance Gene RB/Rpi-blb1. |
| Authors: |
Sorensen, Peyton L.1 (AUTHOR), Christensen, Grace1 (AUTHOR), Karki, Hari S.2 (AUTHOR), Endelman, Jeffrey B.1 (AUTHOR) endelman@wisc.edu |
| Source: |
American Journal of Potato Research. Jun2023, Vol. 100 Issue 3, p240-246. 7p. |
| Subject Terms: |
*LATE blight of potato, *WHOLE genome sequencing, *POTATOES, *PHYTOPHTHORA infestans, *POTATO diseases & pests, *HIGH throughput screening (Drug development), *GENES, *BLIGHT diseases (Botany) |
| Abstract: |
The disease late blight is a threat to potato production worldwide, making genetic resistance an important target for breeding. The resistance gene RB/Rpi-blb1 is effective against most strains of the causal pathogen, Phytophthora infestans. Until now, potato breeders have utilized a Sequence Characterized Amplified Region (SCAR) marker to screen for RB. Our objective was to design and validate a Kompetitive Allele Specific PCR (KASP) marker, which has advantages for high-throughput screening. First, the accuracy of the SCAR marker was confirmed in two segregating tetraploid populations. Then, using whole genome sequencing data for two RB-positive segregants and a diverse set of 23 RB-negative varieties, a SNP in the 5' untranslated (UTR) region was identified as unique to RB. The KASP marker based on this SNP, which had 100% accuracy in the cultivated diversity panel, was used to generate diploid breeding lines containing RB. The KASP marker is publicly available for others to utilize. [ABSTRACT FROM AUTHOR] |
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| Database: |
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