Bibliographic Details
| Title: |
Malaria rapid diagnostic tests reliably detect asymptomatic Plasmodium falciparum infections in school-aged children that are infectious to mosquitoes. |
| Authors: |
Hofer, Lorenz M.1,2,3 (AUTHOR), Kweyamba, Prisca A.3 (AUTHOR), Sayi, Rajabu M.3 (AUTHOR), Chabo, Mohamed S.3 (AUTHOR), Maitra, Sonali L.1,2,3 (AUTHOR), Moore, Sarah J.1,2,3,4 (AUTHOR), Tambwe, Mgeni M.3 (AUTHOR) mmohamed@ihi.or.tz |
| Source: |
Parasites & Vectors. 6/30/2023, Vol. 16 Issue 1, p1-10. 10p. |
| Subject Terms: |
*RAPID diagnostic tests, *SCHOOL children, *MOSQUITO control, *INSECTICIDE resistance, *REVERSE transcriptase polymerase chain reaction, *PLASMODIUM falciparum, *MOSQUITOES |
| Geographic Terms: |
TANZANIA |
| Abstract: |
Background: Asymptomatic malaria infections (Plasmodium falciparum) are common in school-aged children and represent a disease transmission reservoir as they are potentially infectious to mosquitoes. To detect and treat such infections, convenient, rapid and reliable diagnostic tools are needed. In this study, malaria rapid diagnostic tests (mRDT), light microscopy (LM) and quantitative polymerase chain reaction (qPCR) were used to evaluate their performance detecting asymptomatic malaria infections that are infectious to mosquitoes. Methods: One hundred seventy asymptomatic school-aged children (6–14 years old) from the Bagamoyo district in Tanzania were screened for Plasmodium spp. infections using mRDT (SD BIOLINE), LM and qPCR. In addition, gametocytes were detected using reverse transcription quantitative polymerase chain reaction (RT-qPCR) for all qPCR-positive children. Venous blood from all P. falciparum positive children was fed to female Anopheles gambiae sensu stricto mosquitoes via direct membrane feeding assays (DMFAs) after serum replacement. Mosquitoes were dissected for oocyst infections on day 8 post-infection. Results: The P. falciparum prevalence in study participants was 31.7% by qPCR, 18.2% by mRDT and 9.4% by LM. Approximately one-third (31.2%) of asymptomatic malaria infections were infectious to mosquitoes in DMFAs. In total, 297 infected mosquitoes were recorded after dissections, from which 94.9% (282/297) were derived from infections detected by mRDT and 5.1% (15/297) from subpatent mRDT infections. Conclusion: The mRDT can be used reliably to detect children carrying gametocyte densities sufficient to infect high numbers of mosquitoes. Subpatent mRDT infections contributed marginally to the pool of oocyts-infected mosquitoes. [ABSTRACT FROM AUTHOR] |
|
Copyright of Parasites & Vectors is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Complete |
|
Full text is not displayed to guests. |
Login for full access.
|
