Bibliographic Details
| Title: |
Plasma sterols and vitamin D are correlates and predictors of ozone-induced inflammation in the lung: A pilot study. |
| Authors: |
Perryman, Alexia N.1,2 (AUTHOR), Kim, Hye-Young H.3 (AUTHOR), Payton, Alexis2,4 (AUTHOR), Rager, Julia E.1,2,4 (AUTHOR), McNell, Erin E.1 (AUTHOR), Rebuli, Meghan E.1,2,5 (AUTHOR), Wells, Heather2 (AUTHOR), Almond, Martha2 (AUTHOR), Antinori, Jamie2 (AUTHOR), Alexis, Neil E.1,2,5 (AUTHOR), Porter, Ned A.3 (AUTHOR), Jaspers, Ilona1,2,4,5 (AUTHOR) ilona_jaspers@med.unc.edu |
| Source: |
PLoS ONE. 5/15/2023, Vol. 18 Issue 5, p1-20. 20p. |
| Subject Terms: |
*LUNGS, *STEROLS, *PNEUMONIA, *MACHINE learning, *HYDROXYCHOLESTEROLS, *OZONE layer, *OXYSTEROLS, *PILOT projects |
| Abstract: |
Background: Ozone (O3) exposure causes respiratory effects including lung function decrements, increased lung permeability, and airway inflammation. Additionally, baseline metabolic state can predispose individuals to adverse health effects from O3. For this reason, we conducted an exploratory study to examine the effect of O3 exposure on derivatives of cholesterol biosynthesis: sterols, oxysterols, and secosteroid (25-hydroxyvitamin D) not only in the lung, but also in circulation. Methods: We obtained plasma and induced sputum samples from non-asthmatic (n = 12) and asthmatic (n = 12) adult volunteers 6 hours following exposure to 0.4ppm O3 for 2 hours. We quantified the concentrations of 24 cholesterol precursors and derivatives by UPLC-MS and 30 cytokines by ELISA. We use computational analyses including machine learning to determine whether baseline plasma sterols are predictive of O3 responsiveness. Results: We observed an overall decrease in the concentration of cholesterol precursors and derivatives (e.g. 27-hydroxycholesterol) and an increase in concentration of autooxidation products (e.g. secosterol-B) in sputum samples. In plasma, we saw a significant increase in the concentration of secosterol-B after O3 exposure. Machine learning algorithms showed that plasma cholesterol was a top predictor of O3 responder status based on decrease in FEV1 (>5%). Further, 25-hydroxyvitamin D was positively associated with lung function in non-asthmatic subjects and with sputum uteroglobin, whereas it was inversely associated with sputum myeloperoxidase and neutrophil counts. Conclusion: This study highlights alterations in sterol metabolites in the airway and circulation as potential contributors to systemic health outcomes and predictors of pulmonary and inflammatory responsiveness following O3 exposure. [ABSTRACT FROM AUTHOR] |
|
Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Complete |
|
Full text is not displayed to guests. |
Login for full access.
|
