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A short sequence within AluSx induces downstream exon skipping in an ACAT1 minigene model.

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Title: A short sequence within AluSx induces downstream exon skipping in an ACAT1 minigene model.
Authors: Mina Nakama1,2 mnakama@gifu-u.ac.jp, Hiroki Otsuka1,2, Hideo Sasai1,2, Hidenori Ohnishi1,2, Ken-ichirou Morishige1,3
Source: All Life. 2021, Vol. 14 Issue 1, p869-873. 5p.
Subject Terms: *EXONS (Genetics), *ALTERNATIVE RNA splicing, *ANTISENSE RNA, *RNA splicing, *HUMAN genome
Abstract: Alu elements, the most abundant retrotransposed elements in the human genome, are commonly located in introns and affect alternative splicing. We previously showed that an intronic antisense Alu element enhanced alternative splicing in a minigene model. The RNA splicing experiment was performed using a minigene consisting of exons 9–11 of ACAT1 with an antisense AluSx inserted into intron 10 in a pCAGGS vector. Here, we explored the sequence in the intronic antisense AluSx that affects downstream exon skipping. Our RNA splicing analysis with antisense AluSx deletion mutants confirmed a short sequence of 16 bp within the right arm of antisense AluSx that resulted in exon skipping. Our findings help clarify the mechanism of RNA splicing by Alu elements. [ABSTRACT FROM AUTHOR]
Copyright of All Life is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: A short sequence within AluSx induces downstream exon skipping in an ACAT1 minigene model.
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  Data: <searchLink fieldCode="AR" term="%22Mina+Nakama%22">Mina Nakama</searchLink><relatesTo>1,2</relatesTo><i> mnakama@gifu-u.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Hiroki+Otsuka%22">Hiroki Otsuka</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Hideo+Sasai%22">Hideo Sasai</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Hidenori+Ohnishi%22">Hidenori Ohnishi</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Ken-ichirou+Morishige%22">Ken-ichirou Morishige</searchLink><relatesTo>1,3</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22All+Life%22">All Life</searchLink>. 2021, Vol. 14 Issue 1, p869-873. 5p.
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  Data: *<searchLink fieldCode="DE" term="%22EXONS+%28Genetics%29%22">EXONS (Genetics)</searchLink><br />*<searchLink fieldCode="DE" term="%22ALTERNATIVE+RNA+splicing%22">ALTERNATIVE RNA splicing</searchLink><br />*<searchLink fieldCode="DE" term="%22ANTISENSE+RNA%22">ANTISENSE RNA</searchLink><br />*<searchLink fieldCode="DE" term="%22RNA+splicing%22">RNA splicing</searchLink><br />*<searchLink fieldCode="DE" term="%22HUMAN+genome%22">HUMAN genome</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Alu elements, the most abundant retrotransposed elements in the human genome, are commonly located in introns and affect alternative splicing. We previously showed that an intronic antisense Alu element enhanced alternative splicing in a minigene model. The RNA splicing experiment was performed using a minigene consisting of exons 9–11 of ACAT1 with an antisense AluSx inserted into intron 10 in a pCAGGS vector. Here, we explored the sequence in the intronic antisense AluSx that affects downstream exon skipping. Our RNA splicing analysis with antisense AluSx deletion mutants confirmed a short sequence of 16 bp within the right arm of antisense AluSx that resulted in exon skipping. Our findings help clarify the mechanism of RNA splicing by Alu elements. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of All Life is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1080/26895293.2021.1977723
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      – Code: eng
        Text: English
    PhysicalDescription:
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        PageCount: 5
        StartPage: 869
    Subjects:
      – SubjectFull: EXONS (Genetics)
        Type: general
      – SubjectFull: ALTERNATIVE RNA splicing
        Type: general
      – SubjectFull: ANTISENSE RNA
        Type: general
      – SubjectFull: RNA splicing
        Type: general
      – SubjectFull: HUMAN genome
        Type: general
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      – TitleFull: A short sequence within AluSx induces downstream exon skipping in an ACAT1 minigene model.
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          Name:
            NameFull: Mina Nakama
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            NameFull: Hiroki Otsuka
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            NameFull: Hideo Sasai
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            NameFull: Hidenori Ohnishi
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            NameFull: Ken-ichirou Morishige
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              M: 01
              Text: 2021
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              Y: 2021
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              Value: 14
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