Bibliographic Details
| Title: |
A short sequence within AluSx induces downstream exon skipping in an ACAT1 minigene model. |
| Authors: |
Mina Nakama1,2 mnakama@gifu-u.ac.jp, Hiroki Otsuka1,2, Hideo Sasai1,2, Hidenori Ohnishi1,2, Ken-ichirou Morishige1,3 |
| Source: |
All Life. 2021, Vol. 14 Issue 1, p869-873. 5p. |
| Subject Terms: |
*EXONS (Genetics), *ALTERNATIVE RNA splicing, *ANTISENSE RNA, *RNA splicing, *HUMAN genome |
| Abstract: |
Alu elements, the most abundant retrotransposed elements in the human genome, are commonly located in introns and affect alternative splicing. We previously showed that an intronic antisense Alu element enhanced alternative splicing in a minigene model. The RNA splicing experiment was performed using a minigene consisting of exons 9–11 of ACAT1 with an antisense AluSx inserted into intron 10 in a pCAGGS vector. Here, we explored the sequence in the intronic antisense AluSx that affects downstream exon skipping. Our RNA splicing analysis with antisense AluSx deletion mutants confirmed a short sequence of 16 bp within the right arm of antisense AluSx that resulted in exon skipping. Our findings help clarify the mechanism of RNA splicing by Alu elements. [ABSTRACT FROM AUTHOR] |
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| Database: |
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