Bibliographic Details
| Title: |
LINC01224 promotes colorectal cancer progression through targeting miR-485-5p/MYO6 axis. |
| Authors: |
Gu, Jingfeng1 (AUTHOR), Dong, Liang2 (AUTHOR), Wang, Yun3 (AUTHOR), Nie, Wenjia2 (AUTHOR), Liu, Wencong4 (AUTHOR), Zhao, Ji-an1 (AUTHOR) bairenduizhang2020@163.com |
| Source: |
World Journal of Surgical Oncology. 9/17/2021, Vol. 19 Issue 1, p1-12. 12p. |
| Subject Terms: |
*COLORECTAL cancer, *CANCER invasiveness, *REVERSE transcriptase polymerase chain reaction, *MYOSIN, *LINCRNA, *OVERALL survival |
| Abstract: |
Background: Long noncoding RNAs (lncRNAs) are related to colorectal cancer (CRC) development. However, the role and mechanism of lncRNA LINC01224 in CRC development are largely unknown. Methods: LINC01224, Yin Yang 1 (YY1), microRNA (miR)-485-5p, and myosins of class VI (MYO6) levels were examined using quantitative reverse transcription polymerase chain reaction and western blotting. Functional analyses were processed through CCK-8, colony formation, flow cytometry, transwell, and xenograft analyses. Dual-luciferase reporter, chromatin immunoprecipitation (ChIP), RNA immunoprecipitation, and pull-down assays were conducted to analyze the binding interaction. Results: LINC01224 abundance was elevated in CRC tissue samples and cell lines. Elevated LINC01224 might indicate the lower 5-year overall survival in 52 CRC patients. LINC01224 was upregulated via the transcription factor YY1. LINC01224 knockdown restrained CRC cell proliferation, migration, and invasion and increased apoptosis. MiR-485-5p was sponged by LINC01224, and miR-485-5p downregulation relieved the influence of LINC01224 interference on CRC progression. MYO6 was targeted via miR-485-5p and regulated via LINC01224/miR-485-5p axis. MiR-485-5p overexpression suppressed CRC cell proliferation, migration, and invasion and facilitated apoptosis. MYO6 upregulation mitigated the role of miR-485-5p. LINC01224 knockdown decreased xenograft tumor growth. Conclusion: YY1-induced LINC01224 regulates CRC development via modulating miR-485-5p/MYO6 axis. [ABSTRACT FROM AUTHOR] |
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| Database: |
Academic Search Complete |
