Bibliographic Details
| Title: |
Protective Effects of Crataegus pinnatifida Extracts and Crataegolic Acid Against Neurotoxicity of Paraquat in PC12 Cells. |
| Authors: |
Chi-Sen Chang1,2, Yuh-Chiang Shen3,4,5, Chi-Wen Juan6,7, Chia-Lin Chang8 clchang@sunrise.hk.edu.tw, Po-Kai Lin8 |
| Source: |
Current Topics in Nutraceutical Research. 2022, Vol. 20 Issue 1, p76-83. 8p. |
| Subject Terms: |
*MEDICINAL plants, *ANIMAL experimentation, *APOPTOSIS, *CELLULAR signal transduction, *NEUROPROTECTIVE agents, *PLANT extracts, *CELL lines, *CELL surface antigens, *REACTIVE oxygen species, *MICE, *IMMUNODIAGNOSIS, *CASPASES |
| Abstract: |
The neuroprotective mechanisms of Crataegus pinnatifida extracts and crataegolic acid were studied using paraquat induced cytotoxicity in PC12 cells. C. pinnatifida extracts were prepared using hexane, ethyl acetate, and 95% ethanol. Additionally, crataegolic acid (also known as maslinic acid) was found in C. pinnatifida extracts. Assessment methods included the examinations of cytotoxicity, intracellular reactive oxygen species and calcium changes, activity of caspase-3 and a-synuclein, apoptotic cell death, and the expression levels of the B-cell lymphoma 2 (Bcl-2) and BCL2-associated X (Bax) proteins to investigate the neuroprotective mechanisms of C. pinnatifida extracts and its active component, crataegolic acid. The three extracts and crataegolic acid exhibited potent neuroprotective actions against paraquat induced PC12 cell apoptosis at 5--20 μg/mL and 80--100 μM concentrations, respectively. The key protective mechanisms included decreasing cell apoptosis, upregulating Bcl-2 protein levels, and downregulating Bax protein levels. The 95% ethanol extract also decreased paraquat induced reactive oxygen species production, calcium overloading, and caspase-3 and a-synuclein activities. The beneficial effects of these extracts could be explained by the active component, crataegolic acid that also inhibited paraquat-induced apoptosis through the suppression of reactive oxygen species generation and the caspase-3 signaling pathway. [ABSTRACT FROM AUTHOR] |
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| Database: |
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