Bibliographic Details
| Title: |
Identification of CRYAB + KCNN3 + SOX9 + Astrocyte-Like and EGFR + PDGFRA + OLIG1 + Oligodendrocyte-Like Tumoral Cells in Diffuse IDH1-Mutant Gliomas and Implication of NOTCH1 Signalling in Their Genesis. |
| Authors: |
Augustus, Meera1 (AUTHOR) meera.augustus@inserm.fr, Pineau, Donovan1 (AUTHOR) donovan.pineau@igf.cnrs.fr, Aimond, Franck2 (AUTHOR) franck.aimond@inserm.fr, Azar, Safa2 (AUTHOR) safa.azar@ua.edu.lb, Lecca, Davide3 (AUTHOR) davide.lecca@unimi.it, Scamps, Frédérique2 (AUTHOR) frederique.scamps@inserm.fr, Muxel, Sophie1 (AUTHOR) Sophie.Muxel@icm.unicancer.fr, Darlix, Amélie1,4 (AUTHOR) Amelie.Darlix@icm.unicancer.fr, Ritchie, William5 (AUTHOR) william.ritchie@igh.cnrs.fr, Gozé, Catherine1,6 (AUTHOR) c-goze@chu-montpellier.fr, Rigau, Valérie1,7 (AUTHOR) v-rigau@chu-montpellier.fr, Duffau, Hugues1,8 (AUTHOR) h-duffau@chu-montpellier.fr, Hugnot, Jean-Philippe1,9 (AUTHOR) jean-philippe.hugnot@umontpellier.fr, Rostomily, Robert (AUTHOR), Wong, Stephen (AUTHOR), Marosi, Christine (AUTHOR) |
| Source: |
Cancers. May2021, Vol. 13 Issue 9, p2107-2107. 1p. |
| Subject Terms: |
*POTASSIUM metabolism, *CALCIUM metabolism, *PROTEIN metabolism, *NERVE tissue proteins, *GENETIC mutation, *STAINS & staining (Microscopy), *CELL culture, *ONCOGENES, *ION pumps, *EPIDERMAL growth factor receptors, *CELL receptors, *GLIOMAS, *BONE morphogenetic proteins, *CELLULAR signal transduction, *ELECTROPHYSIOLOGY, *DNA-binding proteins, *CELL proliferation, *TRANSCRIPTION factors, *CELL lines, *OXIDOREDUCTASES, *TUMOR markers, *NEUROGLIA, *PLATELET-derived growth factor, *PHENOTYPES |
| Abstract: |
Simple Summary: Diffuse grade II IDH-mutant gliomas are rare brain tumors mainly affecting young patients. These tumors are composed of different populations of tumoral cells. Little is known of these cells and how they are generated. These different cells may show different sensitivity to treatments, so our aim was to study them in detail by directly using patient resections. We identified two clearly distinct tumoral populations and defined reliable markers for them. We also uncovered part of the molecular mechanisms that generate them. Finally, we found that the two cell types have different electrical activity. This article provides unique data and new issues on these rare tumors, which need to be further investigated to develop innovative treatments. Diffuse grade II IDH-mutant gliomas are slow-growing brain tumors that progress into high-grade gliomas. They present intratumoral cell heterogeneity, and no reliable markers are available to distinguish the different cell subtypes. The molecular mechanisms underlying the formation of this cell diversity is also ill-defined. Here, we report that SOX9 and OLIG1 transcription factors, which specifically label astrocytes and oligodendrocytes in the normal brain, revealed the presence of two largely nonoverlapping tumoral populations in IDH1-mutant oligodendrogliomas and astrocytomas. Astrocyte-like SOX9+ cells additionally stained for APOE, CRYAB, ID4, KCNN3, while oligodendrocyte-like OLIG1+ cells stained for ASCL1, EGFR, IDH1, PDGFRA, PTPRZ1, SOX4, and SOX8. GPR17, an oligodendrocytic marker, was expressed by both cells. These two subpopulations appear to have distinct BMP, NOTCH1, and MAPK active pathways as stainings for BMP4, HEY1, HEY2, p-SMAD1/5 and p-ERK were higher in SOX9+ cells. We used primary cultures and a new cell line to explore the influence of NOTCH1 activation and BMP treatment on the IDH1-mutant glioma cell phenotype. This revealed that NOTCH1 globally reduced oligodendrocytic markers and IDH1 expression while upregulating APOE, CRYAB, HEY1/2, and an electrophysiologically-active Ca2+-activated apamin-sensitive K+ channel (KCNN3/SK3). This was accompanied by a reduction in proliferation. Similar effects of NOTCH1 activation were observed in nontumoral human oligodendrocytic cells, which additionally induced strong SOX9 expression. BMP treatment reduced OLIG1/2 expression and strongly upregulated CRYAB and NOGGIN, a negative regulator of BMP. The presence of astrocyte-like SOX9+ and oligodendrocyte-like OLIG1+ cells in grade II IDH1-mutant gliomas raises new questions about their role in the pathology. [ABSTRACT FROM AUTHOR] |
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