Bibliographic Details
| Title: |
Evidence for a role of spindle matrix formation in cell cycle progression by antibody perturbation. |
| Authors: |
Yao, Changfu1, Wang, Chao1, Li, Yeran1, Zavortink, Michael1, Archambault, Vincent2, Girton, Jack1, Johansen, Kristen M.1 kristen@iastate.edu, Johansen, Jørgen1 jorgen@iastate.edu |
| Source: |
PLoS ONE. 11/28/2018, Vol. 13 Issue 11, p1-23. 23p. |
| Subject Terms: |
*EXTRACELLULAR matrix proteins, *DROSOPHILA physiology, *CELL cycle, *IMMUNOGLOBULINS, *MICROTUBULES |
| Abstract: |
In Drosophila it has recently been demonstrated that a spindle matrix in the form of a membrane-less macromolecular assembly embeds the microtubule-based spindle apparatus. In addition, two of its constituents, Megator and Chromator, were shown to function as spatial regulators of spindle checkpoint proteins. However, whether the spindle matrix plays a wider functional role in spatially regulating cell cycle progression factors was unknown. Here using a live imaging approach we provide evidence that a number of key cell cycle proteins such as Cyclin B, Polo, and Ran co-localize with the spindle matrix during mitosis. Furthermore, prevention of spindle matrix formation by injection of a function blocking antibody against the spindle matrix protein Chromator results in cell cycle arrest prior to nuclear envelope breakdown. In such embryos the spatial dynamics of Polo and Cyclin B enrichment at the nuclear rim and kinetochores is abrogated and Polo is not imported into the nucleus. This is in contrast to colchicine-arrested embryos where the wild-type dynamics of these proteins are maintained. Taken together these results suggest that spindle matrix formation may be a general requirement for the localization and proper dynamics of cell cycle factors promoting signaling events leading to cell cycle progression. [ABSTRACT FROM AUTHOR] |
|
Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Complete |
|
Full text is not displayed to guests. |
Login for full access.
|
