Bibliographic Details
| Title: |
Transcriptome profiling and digital gene expression analysis of genes associated with salinity resistance in peanut. |
| Authors: |
Jiongming Sui1, Pingping Jiang2, Guilong Qin2, Shupeng Gai2, Dan Zhu2, Lixian Qiao1, Jingshan Wang1 jswang319@126.com |
| Source: |
Electronic Journal of Biotechnology. Mar2018, Issue 32, p19-25. 7p. |
| Subject Terms: |
*GENE expression, *PEANUT genetics, *SOIL salinity, *GENE ontology, *NUCLEOTIDE sequencing, *PLANT embryology |
| Abstract: |
Background: Soil salinity can significantly reduce crop production, but the molecular mechanism of salinity tolerance in peanut is poorly understood. A mutant (S1) with higher salinity resistance than its mutagenic parent HY22 (S3) was obtained. Transcriptome sequencing and digital gene expression (DGE) analysis were performed with leaves of S1 and S3 before and after plants were irrigated with 250 mM NaCl. Results: A total of 107,725 comprehensive transcripts were assembled into 67,738 unigenes using TIGR Gene Indices clustering tools (TGICL). All unigenes were searched against the euKaryotic Ortholog Groups (KOG), gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, and these unigenes were assigned to 26 functional KOG categories, 56 GO terms, 32 KEGG groups, respectively. In total 112 differentially expressed genes (DEGs) between S1 and S3 after salinity stress were screened, among them, 86 were responsive to salinity stress in S1 and/or S3. These 86 DEGs included genes that encoded the following kinds of proteins that are known to be involved in resistance to salinity stress: late embryogenesis abundant proteins (LEAs), major intrinsic proteins (MIPs) or aquaporins, metallothioneins (MTs), lipid transfer protein (LTP), calcineurin B-like protein-interacting protein kinases (CIPKs), 9-cis-epoxycarotenoid dioxygenase (NCED) and oleosins, etc. Of these 86 DEGs, 18 could not be matched with known proteins. Conclusion: The results fromthis study will be useful for further research on the mechanismof salinity resistance and will provide a useful gene resource for the variety breeding of salinity resistance in peanut. [ABSTRACT FROM AUTHOR] |
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| Database: |
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