| Title: |
Identification of Lablab Semen Album by DNA Barcode Technology. |
| Authors: |
Huiming LUO1,2, Jian RAO1,2 446047751@qq.com, Bingyi XIAO1,2, Ping NIE1,2, Hai LIN1,2, Ye DING1,2 |
| Source: |
Medicinal Plant. Dec2017, Vol. 8 Issue 6, p45-47. 3p. |
| Subject Terms: |
*MEGACOPTA cribraria, *GENETIC barcoding, *POLYMERASE chain reaction, *CHINESE medicine, *NUCLEOTIDE sequence |
| Abstract: |
[Objectives] To identify ITS2 barcode of Lablab Semen Album and its adulterants, and provide a new method for the identification of Lablab Semen Album. [Methods] The ITS2 sequence was amplified by PCR and sequenced bi-directionally. After splicing by CodonCode Aligner, the data were processed with the aid MEGA software to construct the cluster dendrogram (neighbor-joining, NJ tree). [Results] The ITS2 sequence of Lablab Semen Album had length of 218 bp; the constructed cluster dendrogram indicated that aU species were monophyletic and could be distinguished from other species. [Conclusions] The ITS2 barcode can be used for rapid identification of Lablab Semen Album and its adulterants and this experiment further verified that DNA barcode technology is effective in identification of traditional Chinese medicines. [ABSTRACT FROM AUTHOR] |
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| Database: |
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