| Title: |
Single Chain Antibody Fragment against Venom from the Snake Daboia russelii formosensis. |
| Authors: |
Chi-Hsin Lee1 chihsine@msn.com, Yu-Ching Lee2 ycl@tmu.edu.tw, Yueh-Lun Lee1,3 yllee@tmu.edu.tw, Sy-Jye Leu1,3 cmbsycl@tmu.edu.tw, Liang-Tzung Lin1,3 ltlin@tmu.edu.tw, Chi-Ching Chen4 chen3971@landseed.com.tw, Jen-Ron Chiang5 jrc@cdc.gov.tw, Mwale, Pharaoh Fellow6 pmwale@medcol.mw, Bor-Yu Tsai7 boryutsai@navibio.com.tw, Ching-Sheng Hung8 oryx@w.tmu.edu.tw, Yi-Yuan Yang6,9 yangyuan@tmu.edu.tw |
| Source: |
Toxins. Nov2017, Vol. 9 Issue 11, p347. 19p. |
| Subject Terms: |
*RUSSELL'S viper, *SNAKE venom, *IMMUNOGLOBULINS, *ANTIVENINS, *BACTERIOPHAGES, *ENZYME-linked immunosorbent assay |
| Abstract: |
Russell's vipers containing hemotoxic and neurotoxic venom commonly cause snake envenomation. Horse-derived antivenom is a specific antidote, but its production is expensive and has side effects. Developing a cost-effective and more tolerable therapeutic strategy is favorable. In this study, using glutaraldehyde-attenuated Daboia russelii formosensis (DRF) venom proteins to immunize chickens, polyclonal yolk-immunoglobulin (IgY) antibodies were generated and showed a specific binding affinity. Phage display technology was used to generate two antibody libraries of single-chain variable fragments (scFvs) containing 3.4 × 107 and 5.5 × 107 transformants, respectively. Phage-based ELISA indicated that specific clones were enriched after bio-panning. The nucleotide sequences of scFv-expressing clones were analyzed and classified into six groups in the short linker and four groups in the long linker. These scFv antibodies specifically bound to DRF proteins, but not other venom proteins. Mass spectrometric data suggested that these scFv antibodies may recognize phospholipase A2 RV-4 or RV-7. In vivo studies showed that anti-DRF IgY exhibited complete protective effects and mixed scFv antibodies increased the survival rate and time of mice challenged with a lethal dose of DRF proteins. These antibodies can be potentially applied in a rapid diagnostic method or for treatment in the future. [ABSTRACT FROM AUTHOR] |
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