Bibliographic Details
| Title: |
Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation. |
| Authors: |
Haining, Elizabeth J.1 (AUTHOR), Matthews, Alexandra L.1 (AUTHOR), Noy, Peter J.1 (AUTHOR), Romanska, Hanna M.2 (AUTHOR), Harris, Helen J.1 (AUTHOR), Pike, Jeremy1,3 (AUTHOR), Morowski, Martina4 (AUTHOR), Gavin, Rebecca L.1 (AUTHOR), Yang, Jing1 (AUTHOR), Milhiet, Pierre-Emmanuel5 (AUTHOR), Berditchevski, Fedor6 (AUTHOR), Nieswandt, Bernhard4 (AUTHOR), Poulter, Natalie S.7 (AUTHOR), Watson, Steve P.7 (AUTHOR), Tomlinson, Michael G.1 (AUTHOR) m.g.tomlinson@bham.ac.uk |
| Source: |
Platelets. Nov2017, Vol. 28 Issue 7, p629-642. 14p. |
| Subject Terms: |
*TETRASPANIN, *BLOOD platelets, *LABORATORY mice, *NEOVASCULARIZATION, *ANIMAL models in research |
| Abstract: |
The tetraspanins are a superfamily of four-transmembrane proteins, which regulate the trafficking, lateral diffusion and clustering of the transmembrane proteins with which they interact. We have previously shown that tetraspanin Tspan9 is expressed on platelets. Here we have characterised gene-trap mice lacking Tspan9. The mice were viable with normal platelet numbers and size. Tspan9-deficient platelets were specifically defective in aggregation and secretion induced by the platelet collagen receptor GPVI, despite normal surface GPVI expression levels. A GPVI activation defect was suggested by partially impaired GPVI-induced protein tyrosine phosphorylation. In mechanistic experiments, Tspan9 and GPVI co-immunoprecipitated and co-localised, but super-resolution imaging revealed no defects in collagen-induced GPVI clustering on Tspan9-deficient platelets. However, single particle tracking using total internal reflection fluorescence microscopy showed that GPVI lateral diffusion was reduced by approximately 50% in the absence of Tspan9. Therefore, Tspan9 plays a fine-tuning role in platelet activation by regulating GPVI membrane dynamics. [ABSTRACT FROM AUTHOR] |
|
Copyright of Platelets is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Complete |
|
Full text is not displayed to guests. |
Login for full access.
|
